Quisiera agradecer a todas las personas e instituciones que de una u otra forma han contribuido en la realización de este trabajo.Un especial agradecimiento, en primer lugar a mis directores de Tesis: DrC.
RESUMENDentro del género Salmonella, la especie Salmonella enterica, subsp enterica es una de las de mayor interés por su efecto patógeno sobre los animales. El trabajo se realizó con el objetivo de evaluar el efecto in vitro de la acetamida furánica bromada (AFB) sobre Salmonella enterica aviar. La AFB se obtuvo en el Centro de Bioactivos Químicos de la Universidad Central de las Villas, Cuba. La actividad antimicrobiana del producto sobre los aislados de Salmonella entérica se determinó por el método de macrodilución en caldo, según el "National Comité for Clinical Laboratory Standards", NCCLS, documento M7-A2. La concentración letal mínima se determinó según NCCLS, documento M26-A, 1999. Las pruebas bioquímicas y serológicas confirmaron la presencia de Salmonella gallinarum en el 100 % de las muestras. Los valores de la mínima concentración inhibitoria (MCI) y mínima concentración bactericida (MCB) de la AFB fueron ambas de 12.5 mg/ml, para las cepas de Salmonella gallinarum y "American Type Culture Collection" de E. coli. Se concluye que la MCI y MCB de la Salmonella enterica frente a la acetamida furánica bromada in vitro fue de 12.5 mg/ml, demostrando la acción bactericida del producto. ABSTRACT Salmonella enterica subsp. enterica is one of the most interesting bacteria because of its pathogenic effect on animals. The objective of the present study was to evaluate the in vitro effect of furan acetamide bromide (FAB) on avian Salmonella enterica. FAB was obtained from the Centro de Bioactivos Químicos of the Universidad Central de las Villas, Cuba. The antimicrobial activity of the product against Salmonella enterica isolates was determined by the broth macrodilution method, according to the Committee for Clinical Laboratory Standards (NCCLS), document M7-A2. The minimum lethal concentration was determined by NCCLS, document M26-A, 1999. The biochemical and serological tests confirmed the presence of Salmonella gallinarum in 100 % of the samples. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of FAB were 12.5 µg/ml for Salmonella gallinarum and American Type Culture Collection (ATCC) of E. coli. It is concluded that MIC and MBC of Salmonella enterica against in vitro effect of furan acetamide bromide was 12.5 µg/ml, demonstrating the antimicrobial activity of the product.
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