Carbonic anhydrase (CA, EC 4.2.1.1) is a member of the metalloenzyme family. It catalyzes the rapid conversion of carbon dioxide (CO ) and water to bicarbonate (HCO ) and protons (H ) and also plays an important role in biochemical and physiological processes. In this study, a number of novel 2-(4-(aryl)thiazole-2-yl)-3a,4,7,7a-tetrahydro-1H-4,7-methanoisoindole-1,3(2H)-dione derivatives were synthesized and evaluated for their inhibitory characteristics against the human CA isoenzymes I and II (hCA I and hCA II). The structures of the new molecules 8a-i were confirmed by means of IR, H NMR, C NMR, and elemental analysis. These compounds exhibited excellent inhibitory effects, in the low nanomolar range, with K values in the range of 27.07-37.80 nM against hCA I and in the range of 11.80-25.81 nM against hCA II. Our findings suggest that the new isoindolylthiazole derivatives have superior inhibitory effect over acetazolamide (AZA), which is used as clinical CA inhibitor with K values of 34.50 and 28.93 nM against the hCA I and hCA II isoenzymes, respectively.

No abstract

No abstract

In this study, a simple and highly selective potentiometric sensor has been developed for the determination of cobalt(II) ions based on a poly(vinly chloride) (PVC) membrane. As an ionophore, a hydrazone derivative molecule (3) was synthesized for the first time, and its characterization was completed using NMR, FT‐IR and Q‐TOF spectroscopic techniques. The developed sensor works well in the linear concentration range of 1.0×10−6–1.0×10−1 Μ with Nernstian slope of 28.6±1.7 mV/decade of activity. The developed cobalt(II)–selective sensor has a low detection limit of 3.09×10−7 Μ, and a very fast response time of 5 seconds. The sensor, which exhibits a highly selective behaviour towards cobalt(II) ions compared to other metal cations, works in the pH range of 6.0–10.0 without being affected by changes in pH. The developed sensor could be used as an indicator electrode in potentiometric titration of Co(II) ions with EDTA. Finally, the developed cobalt(II)‐selective sensor was successfully used for the determination of Co(II) in various water samples.

No abstract