a Cells (Pseudon lonas ATCC 11250, E. coli K12) were grown in a special flask designed for this type of experiment6 at 25°for about 20 hours with vigorous mechanical shaking. Basal medium contained 0.15% K2HP04, 0.05% KH2P04, 0.02% MgS04-7H20 and 0.1% Difeo yeast extract. In addition, in exp. 1, 0.1% benzoic acid and 0.1% NH4C1; in exp. 2 and 5, 0.1% L-phenylalanine; in exp. 3 and 6, 0.1% L-tryptophan and in exp. 4 and 7, 0.1% glucose and 0.1% NH4C1 were added as carbon and nitrogen sources. The gas phase was a mixture of nitrogen and oxygen in a ratio of 4:1. Highly enriched 0218 gas was prepared by electrolysis of approximately 33% enriched H2018 purchased from The Weizmann Institute of Science, Israel. Cells were harvested by centrifugation, washed with 0.9% KC1 and distilled water. Pyrolysis was carried out at 500°f or 1 hour with HgCl2 as a catalyst according to D. Rittenberg and L. Ponticorvo (Internat. J. Appl. Radiation and Isotopes, 1, 208 (1956)). The mass spectrometric analyses were carried out in collaboration with Mr. W. E. Comstock of this Institute.tions involving fixation of atmospheric oxygen.6-7 When E. coli, a facultative aerobe, was grown under comparable conditions, 018-enrichment in the cell material was approximately 0.1% or less of that of the atmospheric oxygen. Although the data indicate that E. coli also incorporates atmospheric oxygen, particularly when aromatic compounds are used as carbon sources, oxygenases seem to play a more important role in the metabolism of a strictly aerobic microorganism. Further studies are in progress in order to determine the distribution of oxygenases in various tissues and other microorganisms.(5) O.

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The objective of this 6-year retroactive chart review is to compare outcome between chemically dependent physicians and physician assistants under contract with the North Carolina Physicians Health Program (NCPHP). Of 233 physicians 91% had a good outcome, compared to only 59% of 34 physician assistants in this sample (significant by Chi Square method, 99.99% confidence). Fifteen percent of physicians and 37 percent of physician assistants were female with basically the same outcome. Alcohol, followed by opioids, was the predominant substance used by both groups. Most subjects in both groups were between the ages of 30 and 55 with best outcome between the ages of 25-29 and the worst in those over 55. With paucity of data on physician assistants in the literature, the present study may be one of the first to single out this group and compare their recovery rates with those of physicians while receiving similar NCPHP services.

Adaptive increase in histidine decarboxylase activity has been found to occur in all of the three species tested after a variety of stressful stimuli in rat skin following injection of polymyxin B, in mouse skin after burns, in lungs of mice injected with pertussis vaccine and in the skin of guinea pigs during the development of the tuberculin reaction. Mammalian histidine decarboxylase is one of the few enzymes which have been shown to increase in response to nonspecific stress; furthermore the conditions induced by these stressful stimuli have ‘histaminic’ implications. After a discussion of fallacies in earlier studies on the evaluation of the role of histamine, it is concluded that adaptive increase in histidine decarboxylase, attended by slow but increasing formation of histamine, must be seriously considered as an important causative mechanism in slowly developing inflammation.

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