Introduction: Vaccination against varicella-zoster virus (VZV) has been effective and safe in countries that routinely administer the vaccine. Brazil began universal VZV vaccination in 2013. This study aimed to identify VZV genotypes present in Manaus, Brazil prior to widespread immunization. Methods: Vesicular lesions or cerebral-spinal-fluid samples were collected from patients diagnosed with VZV, herpes zoster, or meningitis/encephalitis. DNA was extracted, amplified, and sequenced. Results: Half the isolates were clade-5 viruses and the remaining were divided between the European clades 1 and 3. Conclusions: This study provides insights into the circulating VZV genotypes in Manaus prior to widespread vaccination.