Distemonanthus benthamianus est une plante utilisée en médecine traditionnelle dans certains pays Africains tels que : la Côte d’Ivoire, le Ghana, le Cameroun etc. Plusieurs études ont été réalisées relatives à son effet sur les activités antioxydante, antitumorale, antituberculeuse, bactéricide, antivirale. L’objectif de ce travail a été d’évaluer in vivo l’activité anti-inflammatoire de l’extrait total aqueux des écorces de cette plante. Pour atteindre cet objectif, le screening phytochimique a été réalisé afin de déterminer les métabolites secondaires présentent dans l’extrait de la plante. L’activité antiinflammatoire a été évalué par l’ingestion de la carragénine 1% au niveau l’aponévrose plantaire des rats Wistars. Le screening phytochimique a révélé la présence de tanins, polyphénols, flavonoïdes, saponosides, de triterpènes et d’alcaloïdes Les résultats de l’activité anti-inflammatoire ont montré que le diclofénac et l’extrait aqueux de la plante ont atteint leur activité maximale d’inhibition de l’œdème à la 3ème heure avec une inhibition de 52.88 %, et 44.95%, 60.03% et 70.19 aux doses de 200, 400 et 800mg/kg/pc respectivement. Quant à la prednisone, il a atteint son activité maximale de 26.66% à la 4ème heure. L’inhibition de l’œdème des pattes des rats par l’extrait de la plante montre ainsi les propriétés anti-inflammatoires de cet extrait. L’utilisation de cette plante comme un anti-inflammatoire en milieu naturel serait donc justifiée.

Citrus aurantifolia (Rutaceae) is a plant used in traditional medicine in the treatment of inflammatory and analgesic pathologies. The objective of this study was to evaluate the analgesic activity of the aqueous extract of the leaves of Citrus aurantifolia in mice. The phytochemical study revealed flavonoids, tannins, polyphenols, coumarins, saponosides and polyterpene sterols and the absence of alkaloids. Concerning the acute toxicity study at the single dose of 2000 mg/ Kg pc orally, it revealed that the aqueous extract of the leaves of Citrus aurantifolia is not toxic. Thus, according to OECD guideline 423, the oral LD50 of this extract is in the range of 2000-5000 mg/kg bw. The analgesic activity of the aqueous extract of Citrus aurantifolia orally was determined by evaluating the rate of inhibition of pain caused by acetic acid. The 250 mg/kg bw and 500 mg/kg bw doses of the aqueous extract administered orally all showed analgesic activity. The best analgesic activity was obtained with an inhibition rate of 88.64% for the 250 mg/kg bw dose. The best analgesic activity was obtained with an inhibition rate of 88.64% for the 250 mg/kg bw dose, while with the 500 mg/kg bw dose, the inhibition rate of pain sensation was 47.57%. Therefore, the extract has an analgesic effect at low doses.

Distemonanthus benthamianus is a plant of the Caesalpiniaceae family, used in traditional medicine to treat inflammatory problems such as rheumatism, bronchitis, epilepsy, and boils. The objective of the present work is to develop the pharmacological properties of this plant by evaluating the antioxidant (in vitro) and anti-arthritic properties of the bark of the plant on wistar rat. The antioxidant activity of the extract was evaluated by determining the antiradical activity through the DPPH radical scavenging test and the reducing activity of the extract. The anti-arthritic activity of the extract was evaluated on Wistars rats. Arthritis was induced in rats by injection of Freud's complete adjuvant in the plantar fascia of the left leg of the rats. The reducing power and the IC50 of the extract were 426.0±17.31 µmol Eq/Trolox/g EXS and 26.67±1.12 µg/mL, respectively. The plant extract and reference molecules administered to arthritic rats significantly reduced edema and arthritic signs. The weight of the rats was quickly stabilized at the level of the treated rats. At the end of the experiment, the percentages of inhibition of the extract at the doses of 200, 400, 800 mg/kg/bw were respectively 38.2%, 44.33% and 48%. As for the molecules of diclofenac sodium and Prednisone (5 mg/kg/bw), the percentages of inhibition were 47.33% and 37.84% respectively. A part from the reduction of edema, the hematological (leukocytes, platelets) and biochemical (fibrinogen, total protein, albumin, total and conjugated bilirubin, AST, ALT) parameters evaluated in treated rats showed no significant difference compared to healthy rats. These results confirm the traditional use of Distemonanthus benthamianus bark in cases of chronic inflammatory diseases.

Allergic diseases are constantly growing, however the efficiency of classical treatments is not total. Thus, new therapeutic tools are considered such as phytotherapy. The objective of this study was to demonstrate the effect of the aqueous extract of Moringa oleifera in mice. The phytochemical study revealed the presence of poly terpenes/sterols, polyphenols, flavonoids, tannins and alkaloids but also the absence of quinones and saponosides. The acute toxicity study at a single dose of 2000 mg/kg body weight (bw) by the oral route revealed that the aqueous extract of Moringa oleifera is not toxic and would have an oral LD50 greater than to 2000 mg/kg bw. The anti-allergic effect of Moringa oleifera leaf Total Aquous Extract (E.T.A.) was evaluated by observing the number of scratching in allergic mice treated orally with two different doses of this extract. A reduction of the number of scratching in mice treated with the extract was observed. This was confirmed by hematological analysis where a considerable increase in the number of immune cells and a decrease in the recruitment of these cells to inflammatory sites were observed. This confirms that the aqueous extract of Moringa oleifera has a dose-dependent antiallergic activity

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