The purpose of this study is to access the level of knowledge, training and compliance of these house officers to universal precautions to HIV transmission. Study Design: Cross Sectional study. Setting: Aziz Bhatti Shaheed Teaching Hospital Gujrat. Period: July-September 2019. Material & Methods: Self reported questionnaires were provided to 80 house officers and results were analyzed in SPSS 20. Results: knowledge attitude and compliance of house officers towards universal precautions was not up to date. This could be due to the fact that 78.79% of our participants have not received any form of training regarding universal precautions. Conclusion: It is urgently needed that training sessions for newly recruited doctors are arranged on regular basis to prepare them protect themselves against communicable diseases. Also strategies should be devised to implement strict compliance to universal precautions.