Aim: This study was carried out to evaluate the personality traits, professional self-concept and occupational perceptions of the nurses and to examine the relationship among them. Method: This is a descriptive and correlational study. The research sample was made of 185 nurses working in two private hospitals affiliated to a foundation university and in a medical center located in Istanbul Province. Data were obtained using the Ten-Item Personality Scale, the Perception of Nursing Profession Scale and the Professional Self-Concept Scale in Nurses. Results: The average age of nurses was 25.32, 42.2% had bachelor's degree and the duration of experience as a nurse was 5.21 years. In the ranking of the average scores of the answers given to the items of the Ten-Item Personality Scale, the averages of the statements "I consider myself as reliable self-disciplined", "I consider myself as sympathetic, warm-hearted" and "I consider myself as extroverted, enthusiastic" were higher than the means of other items. The general perceptions of nurses on nursing profession are moderately positive. Scores showed that professional self concept in nurses was quite positively (110.30±12.53). Positive relationship was found between the scores of Extroversion sub-dimension of the Ten-Item Personality Scale and the Professional Satisfaction sub-dimension scores of the Professional Self-Concept Scale in Nurses (p=0.006). No statistically significant relationship was found between the scores of the Ten-Item Personality Scale and Perception of Nursing Profession Scale (p>0.05). Statistically significant positive relationship was determined between scores of the Perception of Nursing Profession Scale and the Professional Self-Concept Scale in Nurses (p<0.01). Conclusion: Research results show ed a positive relationship between nurses’ occupational perceptions and professional self-perceptions, and that there is a need for strategies to improve the nurses’ occupational perceptions. It is important to adopt approaches that improve occupational perceptions and professional self-perception in accordance with the personality traits.