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The aim of the present study was to test the psychometric properties of the Aggression Questionnaire Short Form for adolescents and adults in Turkish. The adaptation study was conducted with 778 adolescents aged between 15-18 and 1067 adults aged between 19 and 44. The construct validity of the questionnaire was tested via Parallel Analysis, Exploratory Factor Analysis and Confirmatory Factor Analysis. Furthermore, item-total correlations, test-retest score correlation, and internal consistency (Cronbach Alpha and McDonald's Omega) were calculated as reliability analyses. The Measurement Invariance test and Differential Item Functioning in male and female, adolescent and adult samples were also conducted. The results yielded that the Turkish version of the Aggression Questionnaire Short Form is a reliable questionnaire with four-factors, and without sex and age differences, it can be used to measure aggression among Turkish adolescents and adults.