The soil seed bank is an important natural regeneration strategy for plant communities and can determine floristic composition after disturbances. The aim of the present study was to evaluate the seed bank richness and abundance at different soil depths and under different light conditions in a dry forest. Litter and soil samples were collected at depths of 0-5 and 5-10 cm and submitted to two light conditions (light and shady). In total, 1,725 individuals from 85 species and 19 families emerged. Significant differences in richness between soil depths were observed, being greater at 0-5 cm, while abundance was similar. There were no variations in richness or abundance of germinated seeds between light conditions. Malvaceae and Verbenaceae families were the most representative in this study.

Chagas disease (CD) is still a neglected disease. Infected individuals are diagnosed late, being treated in worse clinical conditions. Thus, this study aimed to analyze the prevalence and the factors associated with new confirmed cases of CD identified by serological screening in an endemic region of Minas Gerais State, Brazil. This is an analytical cross-sectional study with data from a project of the Research Center in Tropical Medicine of Sao Paulo- Minas Gerais (SaMi-Trop) conducted in two municipalities. Data collection included a questionnaire with closed questions, a venous blood collection and an ELISA serological test for CD. A total of 2,038 individuals with no previous diagnosis of CD participated in the study. The result of the serological test for CD was adopted as the dependent variable. The independent variables addressed personal issues, health conditions and lifetime housing. A descriptive analysis of individual variables was performed. Subsequently, a bivariate analysis was performed using the Pearson’s chi-square test. Households sheltering individuals positive for CD were georeferenced, and the analysis of spatial distribution was performed using the quartic function to estimate the density of the nucleus. Among the participants, 188 (9.2 %) were positive for CD. The profile of participants with CD was associated with place of residence, age, relative/family member with CD and living conditions. It is noteworthy that there are still patients with CD who are unaware of their diagnosis in both, rural and urban areas.