Effects on the tissue mineral concentrations of pigs from sows fed four dietary Zn levels were studied. A male and a female from first- and second-parity litters were killed at 1 and 21 d of age. The dams were fed a corn-soybean meal basal diet supplemented with 0, 50, 500 or 5,000 ppm Zn from 30 kg body weight until completion of the second lactation. Pigs from sows fed 5,000 ppm additional zinc had heavier liver, heart, thyroid and adrenal weights relative to their body weight than did pigs from sows on the other treatments. First- and second-parity pigs from sows on the highest Zn supplementation level had higher Fe stores in the liver, higher Zn concentrations in the liver, kidney and pancrease, and higher Cu levels in the kidney compared with pigs from sows on the other treatments. However, Cu concentrations in the liver, heart, pancreas, esophagus, aorta and testes were reduced in pigs from sows on the 5,000 ppm Zn treatment. In first-parity pigs, Ca in the liver was higher for pigs whose dams received 5,000 ppm Zn compared with pigs from sows on all other treatments, and the Mn level was higher compared with pigs from sows receiving 50 or 500 ppm additional zinc. Pigs at 1 d of age from sows on the 0, 50 or 500 ppm treatment had lower hepatic P and Zn concentrations than pigs from sows on the same treatment at 21 d of age. The reverse was true for pigs whose dams received 5,000 ppm Zn.
A previously reported method for selenium analysis of biological materials has been modified to reduce equipment requirements and labor, resulting in 40—80 determinations in an 8-hr period. Digestions are performed on hot plates in Erlenmeyer flasks, and neutralization, chelation with EDTA, complexing with 2,3- diaminonaphthalene, and extraction of the piazoselenol into cyclohexane are completed in the same vessel. Flotation of the cyclohexane layer into the neck of the flask with water allows convenient transfer to fluorometer tubes. Representative analytical values for serum, skeletal muscle, liver, kidney, corn, and alfalfa hay are presented. The mean recovery (± standard deviation) of added selenite selenium in 84 determinations was 98.1±7.1%. The mean coefficient of variation (± standard error) of repeated analyses of the same samples was 6.98±0.78%. The mean difference (± standard error) between values determined by the proposed method and the AOAC method was -0.03±0.60%.
Fifteen second-parity sows were used to determine the importance of vitamin E (E) and selenium (Se) supplementation of the sow's diet and colostrum consumption by the neonatal pig on tolerance to parenteral iron. Selenium (.1 ppm) and E (50 IU/kg) supplementation of the diet of the sow increased plasma tocopherol and Se concentrations, but did not increase plasma glutathione peroxidase (GSH-Px) activity. Colostrum had greater concentrations of E (primarily alpha-tocopherol) and Se than milk. Plasma biological antioxidant status (tocopherol level and GSH-Px activity) of pigs at birth was very low, but by 2 d of age had increased, especially in alpha-tocopherol (nearly a 20-fold increase). Liveability and body weight gain of pigs were not affected by the pre-colostrum iron injection (200 mg Fe as gleptoferron); however, plasma tocopherol concentrations of Fe-injected pigs were lower and plasma Se concentration and GSH-Px activities were higher at 2 d of age than values of pigs not receiving parenteral Fe. Supplementation of the dam's diet with E and Se maintained high tocopherol and Se levels in her colostrum and milk and a high biological antioxidant status in her pigs throughout the nursing period.
The acceptability and digestibility of a high-fiber biscuit-based diet was investigated using two adult male Colobus guereza animals. Although the animals were initially reluctant to accept the biscuit, it was eventually readily consumed. Apparent digestion coefficients for the diet (average composition, dry matter basis: 16% crude protein, 25% neutral detergent fiber (NDF), 9.5% acid detergent fiber (ADF), 1.2% acid lignin) determined by total fecal collection were 0.871 for dry matter, 0.813 for NDF, 0.693 for ADF, and 0.208 for acid lignin. Fiber digestive capabilities in C. guereza generally exceeded those reported in ruminant species based on predictive equations. Use of acid lignin and Crz03 as markers underestimated dry matter digestibility by 3.9 and 6.0%, respectively.
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