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The fatigue behavior of the negative moment region of continuous steel–concrete composite beams under Canadian temperature conditions was studied. Tests were conducted on three 26 ft 0 in. (7.92 m) long beams, continuous over a central support, and on 11 conventional push-out specimens. These were supplemented by a theoretical study of the internal forces in the beams using an iterative method of analysis.The close agreement between measured and theoretical strains and deflections indicated that good interaction was achieved throughout the length of the beams. The beams sustained 500 000 cycles of loading with no serious deterioration of composite action. The pattern of stud failures was consistent from beam to beam and reflected closely the calculated distribution of horizontal shear force at the steel–concrete interface. Stud shear connectors in the negative moment region where the slab had cracked in tension were found to be slightly less effective than studs in the positive bending moment regions.Neither the detailed study of individual connectors in the beams nor the results of the push-out tests show evidence of a reduction in the fatigue life of studs at −20 °F (−29 °C) relative to room temperature. The beams also exhibited no significant difference in their overall performance at these two temperatures.