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601) 232-5353 BOOK REVIEW BIOLOGICAL EFFECTS OF ELECTROMAGNETIC RADIATION e d i t e d by John M. Osepchuk. IEEE Press, 1983, 608 pages, member p r i c e $47.95.

The growing interest in the physiology of lymphocytes caused the need for obtaining pure suspensions of these cells from peripheral blood. Numerous ingenious methods were devised t o this end [5]. I n the course of our experiments on lymphocyte cultivations in vitro none of these methods was found satisfactory for routine use, not in our hands a t least.Most promising simple methods previously described [2, 41 were tested and finally a routine procedure was developed which fulfilled the following requirements :(1) The suspension should be contaminated as little as possible with other cells.(2) The apparatus which is necessary must be as simple as possible.(3) The handling of the cells must be limited t o a minimum. (4) The maintenance of sterile conditions should be easily possible. (5) No cytotoxic agents must be introduced. Basic principles. Filtration of leukocyte suspensions through glass wool columns [2] or sedimentation in glass containers [4] eliminates platelets, granulocytes and monocytes due t o their adhesion t o foreign surfaces. The observation of the phenomen of spreading out (Etalement spontan6e of BESSIS [l]) indicates that the process of separation may be enhanced by the use of plastic surfaces. Formwar and metacrylate were tried by us. MethodsLeukocytes and platelets suspended in own plasma were obtained from human or rabbit heparinized venous blood (10 I.U. per 10ml) by

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