RESUMO -Microalgae are potential sources of high value chemicals and bioactive compounds. To promote the cell productivity, culture conditions such as carbon and nitrogen sources, irradiance, temperature, bioreactor design have been investigated. Under mixotrophic conditions, some microalgae grows rapidly and to have a higher growth rate when compared to photoautotrophic conditions. This works reports Chlorella vulgaris production under mixotrophic conditions using glycerol and corn steep licor. The factorial design (2 2 plus star central) was performed with 0.5-1.5% gycerol (C gly ) and 0.5-1.5% corn steep liquor (C csl ) using maximum cell concentration (X m ), cell productivity (P X ) as response. Results from the response surface methodology were that the X m of 1.52 g L -1 was obtained using 0.9 % of G and 1.2 % of CSL. The highest P X value was 232 mg L -1 day -1 with 1.5% of G and CSL. Therefore, these models can be useful to predict maximum cell concentration in mixotrophic cultures.