This study was undertaken to evaluate neurodevelopmental outcome of children at 6 years of age after intrauterine laser therapy for Twin-twin transfusion syndrome (TTTS). This is part of a longitudinal study in children after intrauterine laser therapy for TTTS; 190 of 254 (74.8%) children, previously investigated at a median age of 2 years 10 months, were re-evaluated at 6 years 5 months (range 4 years 11 months -10 years 4 months). Sixty-four patients were not examined due to loss of contact. The median gestational age at birth was 34 + 3 weeks. The study included a physical/neurological examination, a standardized neurodevelopmental test (Kaufman-ABC) and/or results from the national screening programme for children as well as questionnaires. Patients were grouped in three outcome categories: group I: normal examination and test result; group II: minor neurological deficiencies and normal test results; group III: major neurological deficiencies and/or test results below minus two standard deviations. The following results were obtained at 6 years 5 months (for comparison, results of the same patients at 2 years 10 months in brackets). Group I: 79.5% (84.2%); group II: 11.6% (8.9%); group III: 8.9% (6.8%). Twenty-one (11%) patients had a worse and 8 (4.2%) an improved classification at 6 years 5 months as compared to 2 years 10 months. Overall, the results with 6 years did not significantly differ from the results with 2 years. Neurodevelopmental outcome at 6 years 5 months was not statistically, significantly different from outcome at 2 years 10 months.

Despite severe prenatal cardiac involvement, childhood cardiac function is normal in the majority of surviving donors and recipients after successful LC of severe TTTS. This underlines the favourable impact of intrauterine LC on postnatal cardiovascular performance.

The ureterovesical junction was studied by dissection and serial sections in 50 post mortem specimens. Three points are considered in this paper: the structure of the terminal ureter, the anatomical arrangement of the ureteral hiatus and the ureteral sheath. Study of the structure of the intramural segment of the ureter demonstrated on one hand, the perfect continuity of the terminal ureter with the trigone and on the other hand, the abundance of the fibroelastic connective fibers which like the muscle fibers run longitudinally. The compliance of the intravesical ureter is dependent on the balance between these two components. The modification of this balance can lead to the creation of a functional obstacle. The anatomical arrangement of the ureteral hiatus is described. The inner muscle layer of the detrusor extends almost to the ureteral orifice, the truly submucosal part of the ureter thus being very small. The constitution of the ureteral orifice and its relations to the ureter account for the different positions of juxtaureteral diverticula and transhiatal herniae of the bladder mucosa. The many descriptions of the ureteral sheath appearing in the literature are reviewed in light of the findings from the present study. The juxtavesical segment of the ureter is surrounded by a fibroconjunctive sheath which fixes the ureter to the bladder wall. The transparietal segment of the ureter is ensheathed in its adventitia, whereas a fibromuscular sheath cannot be truly individualized over this ureteral segment.

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Methods: 16 New Zealand pregnant rabbits were included in the placental insufficiency (PI) model, in which a 40-50% of uteroplacental vessels were ligated at 25d of gestation. Contralateral horn were considered as control. After surgery, dams were allowed free access to water and standard chow. 2 New Zealand pregnant rabbits were included in the hyponutrition (HN) model, in which a sham-surgery was performed at 25d. After surgery, hyponutrition was induced by restricting up to 70% of the normal diet. Pulsed Doppler assessment was performed before Cesarean delivery. Results: Mortality rate showed significant differences between experimental groups (14,3% control; 5.0% HN; and 54.2% PI (p<0.001)). Among survivors, all biometric parameters showed a consistent and significant gradient across the experimental groups. Doppler assessment demonstrated a significant linear increase of ductus venosus and a linear decrease of umbilical vein blood flow across study groups. Additionally, umbilical artery and aortic isthmus showed a non-significant trend to increase through experimental groups. Conclusion: Selective ligature of uteroplacental vessels results in an animal model of fetal growth restriction with biometric and hemodynamic changes that closely resembles the clinical condition. This surgical model could be useful to investigate effects of hypoxic condition on different key organs, such as brain and heart.