Pseudomonas is an invasive organism that causes severe Hospital Acquired Infections in immune compromised hosts and it exhibits a high degree of resistance to broad spectrum antibiotics. In recent years Colistin is the drug of choice for use against MDR strains. The aim was to study the antibiotic susceptibility and Colistin sensitivity pattern of Pseudomonas isolates from various samples. Material and methods: 100 Pseudomonas aeruginosa isolates from various samples (exudates, bloods and urines) were included and other isolates were excluded in the study. The samples were collected under aseptic precautions. Isolation and identification of the isolates was done as per the standard guidelines in the lab and AST was done by Kirby Bauer disc diffusion method and interpretation was done as per CLSI guidelines. Results: Out of 100 isolates, 36 were from the surgical wards;30, 20 and 14 were from medical wards, OBG and GHCCD respectively. 83%of isolates were sensitive to Colistin and 17% were resistant. MDR strains (both ESBL's and MBL's) were detected in 33% and out of these 15.2% were also resistant to Colistin. Conclusion: Colistin is one of the first antibiotics with significant activity against MDR Gram negative pathogens and its usefulness has been documented that it will be the "last line" therapeutic drug in the 21 st century. In the present study it is showing that resistance to Colistin is also increasing, recommending regular monitoring of AST for proper management and to limit its use routinely.

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