Mastitis is the most frequent disease of dairy cows and has well-recognized detrimental effects on animal wellbeing and dairy farm profitability. Since the beginning of modern dairy farming, producers have sought effective methods to minimize the occurrence of mastitis in their herds. The objective of this paper is to review and highlight important advances in detection, management, and prevention of mastitis that have occurred since the first volume of the Journal of Dairy Science was published in 1917. Initial research efforts were directed at understanding the nature of pathogenic bacteria that were responsible for most intramammary infections. For decades, researchers worked to identify effective strategies to control mastitis caused by Streptococcus agalactiae and Staphylococcus aureus. To develop successful control programs, mastitis workers first had to identify mechanisms of infection, define the clinical and subclinical states of the disease, discover appropriate screening tests, determine likely points of exposure, identify pathogen-specific characteristics, and develop effective procedures for machine milking. Pioneering researchers eventually recognized that mastitis control was based on preventing new infections from occurring in healthy cows and reducing the duration that cows remained infected. Development of a control program that incorporated post-milking teat dipping, hygienic milking procedures, and strategic use of antibiotic therapy at dry-off resulted in widespread control of contagious pathogens. As herd management changed, researchers were tasked with defining control of mastitis caused by opportunistic pathogens originating from environmental sources. As mastitis pathogens have evolved, researchers have sought to define antimicrobial usage that will maintain animal wellbeing while minimizing unnecessary usage. During the last century, tremendous significant advances in mastitis control have been made but changing herd structure and more rigorous processor standards ensure that mastitis will remain an important subject focus of future research.

The objective of this study was to develop a method to quantify antimicrobial drug usage and treatment practices on conventional and organic dairy farms that had been recruited to represent a broad spectrum of potential exposure to antimicrobial drugs. Data on disease prevalence and treatment practices of organic (n = 20) and conventional (n = 20) farms were obtained during a farm visit using a survey instrument. A standardized estimate of antimicrobial drug usage was developed using a defined daily dose (DDD) of selected compounds. Density of antimicrobial drug usage was expressed as the number of DDD per adult cow per year. Differences in prevalence and management of selected diseases between conventional and organic farms were identified. The overall estimated prevalence of selected diseases was greater for conventional farms compared with organic farms. Organic farmers reported use of a variety of nonantimicrobial compounds for treatment and prevention of disease. Conventional farmers reported that penicillin was the compound most commonly used for dry cow therapy and cephapirin was most commonly used for treatment of clinical mastitis. On conventional farms, the estimated overall exposure to antimicrobial drugs was 5.43 DDD per cow per year composed of 3.58 and 1.85 DDD of intramammary and parenteral antimicrobial drugs, respectively. Of total intramammary antimicrobial drug usage, treatment of clinical mastitis contributed 2.02 DDD compared with 1.56 DDD attributed to the use of dry cow therapy. Of total parenteral treatments, the distribution of exposure was 0.52 (dry cow therapy), 1.43 (clinical mastitis treatment), 0.39 (treatment of foot disease), 0.14 (treatment of respiratory disease), and 0.32 (treatment of metritis) DDD. For treatments of foot infections (0.33 DDD), respiratory infections (0.07 DDD), and metritis (0.19 DDD), the mean density of ceftiofur usage was significantly greater compared with other compounds.

The objective of this study was to determine the relationship between udder and leg hygiene scores of lactating dairy cattle and measures of subclinical mastitis. Study animals (n = 1250) consisted of lactating dairy cows from eight commercial dairy farms. Herds were enrolled during December 2000 and January 2001 and were visited bimonthly for a total of five visits per herd. Udder and leg hygiene scores were recorded by one person using a four-point scale ranging from one (very clean) to four (very dirty). Udder and leg hygiene scores were compared to bacteriological cultures of milk samples and monthly individual SCC values. Mean hygiene scores were 2.09 and 2.33 for udders and legs, respectively. Udder hygiene scores (UHS) were significantly associated with leg hygiene scores and varied among farms. Linear somatic cell scores increased as udder hygiene score increased. Significant differences in somatic cell scores were observed for all contrasts of udder hygiene score, except between scores of 1 and 2 and of 3 and 4. Linear somatic cell scores were associated with leg hygiene scores, but the only significant contrast was between leg hygiene scores of 2 and 4. There was a significant association between the prevalence of intramammary contagious pathogens and udder hygiene score. The prevalence of intramammary environmental pathogens was significantly associated with udder hygiene score and was 7.7, 10.0, 10.6, and 13.5% for UHS of 1, 2, 3, and 4, respectively. The prevalence of environmental pathogens was not associated with LHS. Cows with udder hygiene scores of 3 and 4 were 1.5 times more likely to have major pathogens isolated from milk samples compared with cows with hygiene scores of 1 and 2.

In recent years, the US dairy industry has experienced significant demographic changes, with an increase in the number of large herds. The objectives of the present study were to characterize clinical mastitis occurring in cows on large dairy herds in Wisconsin. Participating herds (n=50) were required to have a minimum of 200 lactating animals, participate in monthly DHI testing (including monthly individual cow somatic cell count), use computerized herd records, use a milking routine that included fore-stripping quarters for detection of mastitis, and use antimicrobials to treat affected cows. After study personnel visited the farm, each herd was instructed to enroll the next 17 cows that experienced clinical mastitis, regardless of severity. At detection of clinical mastitis and 14 to 21 d after treatment ended, duplicate quarter milk samples were collected from all affected quarters and used for microbiological analysis. Treatments of affected cows were performed according to existing individual farm protocols. Cow level follow-up data was collected for 90 d after enrollment. Microbiological diagnoses at enrollment included gram-negative (35.6%), no growth (27.3%), gram-positive (27.5%), and other (9.6%). Of the 741 cases, the most prevalent pathogens were Escherichia coli (22.5%), followed by environmental streptococci (12.8%), Klebsiella spp. (6.9%), and coagulase-negative staphylococci (6.1%). Bacteriological cure was 75.0% for cases caused by gram-negative pathogens (n=136), 50.8% for cases caused by gram-positive pathogens (n=128), 47.5% for cases caused by other pathogens (n=40), and 73.2% for cases which did not result in microbial growth (n=123). Of the 583 cases with severity recorded, the distribution of mild, moderate, and severe symptoms was 47.8, 36.9, and 15.3%, respectively. The majority of cases presenting with severe symptoms were caused by gram-negative pathogens. Treatment cure was greater for gram-negative pathogens and cases for which no pathogens were recovered as compared with cases caused by other etiologies. Cows experiencing severe cases were more likely to receive multiple antimicrobial treatments.

The primary objective was to compare reported antimicrobial usage between conventional and organic dairy farms. A secondary objective was to contrast selected management characteristics of conventional and organic dairy herds. A questionnaire was administered on site to selected dairy farmers located in Michigan, Minnesota, New York, and Wisconsin. Organic herds (n = 32) were smaller and produced less milk than conventional herds (n = 99). Lactating cows in organic dairies were more likely to be housed in tie stalls, whereas most conventional dairies housed cows in free stalls and milked in a parlor. Total mixed rations and purchased feeds were used on more conventional dairy farms compared with organic dairy farms. Conventional dairy producers were more likely to use advice from veterinarians for recommendations of treatment, and organic dairy producers were more likely to rely on advice from other farmers. Based on recall of antibiotic usage in the previous 60 d, 5.1, 84.9, 9.1, and 0.9% of farmers with conventional herds reported treatment of none, 1 to 10%, 11 to 25%, and >25% of milk cows, respectively. Most organic farmers (90.6%) reported no antibiotic treatments of milk cows, whereas 9.4% reported treating 1 to 10% of milk cows. Ceftiofur was the most commonly reported antibiotic for both farm types. Milk replacer containing antibiotics was reportedly used on 49.5% of conventional herds but only on one organic herd (3.1%). Antibiotics were used in heifer calves on 74.7% of conventional herds versus 21.9% of organic herds. Antibiotics to treat mastitis were used on 79.8% of conventional herds but on none of the organic herds. Most organic farms were in compliance with standards in advance of implementation of regulations.