Implementation of tubular endothelial cell networks is a prerequisite for 3D tissue engineering of constructs with clinically relevant size as nourishment of cells is challenged by the diffusion limit. In vitro generation of 3D networks is often achieved under conditions using serum containing cell culture medium and/or animal derived matrices. Here, 3D endothelial cell networks were generated by using human umbilical vein endothelial cells (HUVECs) in combination with human adipose tissue derived stromal cells (hASCs) employing human collagen I as hydrogel and decellularized porcine small intestinal submucosa as starter matrix. Matrigel/rat tail collagen I hydrogel was used as control. Resulting constructs were cultivated either in serum-free medium or in endothelial growth medium-2 serving as control. Endothelial cell networks were quantified, tested for lumen formation, and interaction of HUVECs and hASCs. Tube diameter was slightly larger in constructs containing human collagen I compared to Matrigel/rat tail collagen I constructs under serum-free conditions. All other network parameters were mostly similar. Thereby, the feasibility of generating 3D endothelial cell networks under serum-free culture conditions in human collagen I as hydrogel was demonstrated. In summary, the presented achievements pave the way for the generation of clinical applicable constructs.
This study investigated trends in performance and sex difference in swimming speed of elite open-water swimmers at FINA 10 km competitions (i.e. World Cup races, European Championships, World Championships and Olympic Games). Swimming speed and sex difference in swimming speed of the fastest and the top ten women and men per event competing at 10 km open-water races between 2008 and 2012 were analysed using single and multi-level regression analyses. A total of 2,591 swimmers (i.e. 1,120 women and 1,471 men) finished 47 races. Swimming speed of the fastest women (1.35 ± 0.9 m/s) and men (1.45 ± 0.10 m/s) showed no changes across years. The mean sex difference in swimming speed for the fastest swimmers was 6.8 ± 2.5%. Swimming speed of the top ten female swimmers per event was 1.34 ± 0.09 m/s and remained stable across the years. The top ten male swimmers per event showed a significant decrease in swimming speed over time, even though swimming speed in the first race (i.e. January 2008, 1.40 ± 0.0 m/s) was slower than the swimming speed in the last race (i.e. October 2012, 1.50 ± 0.0 m/s) (P < 0.05). To summarize, swimming performances remained stable for the fastest elite open-water swimmers at 10 km FINA competitions between 2008 and 2012, while performances of the top ten men tended to decrease. The sex difference in swimming speed in elite ultra-swimmers (~7%) appeared smaller compared to other ultra-distance disciplines such as running. Further studies should examine how body shape and physiology of elite open-water ultra-distance swimmers influence performances.
The basal amounts of metallothionein (MT) and its rates of biosynthesis were compared in resting and proliferating Chang liver (CCl-13) cells. In resting cells the total amounts of the detectable isoforms MT-2 and MT-1e were approx. 1.6x10(6) and 4x10(5) molecules per cell respectively. In exponentially growing cultures the cellular contents of both isoforms increased co-ordinately approx. 4-fold and decreased again to the initial values within 48 h after entering density-mediated growth arrest. As documented for MT-2 its transient accretion was attributable to a 10-fold rise in the rate of biosynthesis of this protein during the growth phase. Measurements of the relative amounts of MT-2 mRNA indicated the occurrence of a more than 50% increase within the first 12 h after subculturing of the cells, followed by a return to basal levels thereafter. These results denote a direct link between the programming of MT synthesis and proliferation and thus attest to a central housekeeping function of the MTs.
Competitive swimming is a reasonable safe sport, where overuse injuries are predominant. 200 - 400 m breaststroke events increase the risk for knee overuse injuries more than other disciplines. Training for more than four times a week increases the risk twofold for knee and fourfold for shoulder overuse injuries.
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