A simple and efficient protocol is described for regeneration of wild sorghum (Sorghum dimidiatum) from cell suspension cultures. Fast-growing cell suspensions were established from shoot-meristem-derived callus. Plating of the suspension on Murashige and Skoog agar medium supplemented with 2.5 mg l -1 2,4-dichlorophenoxyacetic acid (2,4-D) resulted in the formation of embryogenic calli. High-frequency (80%) somatic embryogenesis from small cell clusters (300-400 µm) was observed when the cultures were initially maintained in liquid medium with reduced levels of 2,4-D (0.25 mg l -1 ), followed by transfer to regeneration medium. Direct plating of these small clusters on regeneration medium or transfer to liquid regeneration medium containing kinetin and 6-benzylaminopurine resulted in the development of mature somatic embryos and plantlets. The regenerants developed to maturity and were all phenotypically and cytologically normal.Abbreviations BAP 6-Benzylaminopurine · 2,4-D 2,4-Dichlorophenoxyacetic acid · MS Murashige and Skoog Materials and methods Establishment of cell suspensionsThree-month-old rapidly growing friable yellowish embryogenic calli were used to initiate cell suspension cultures. The calli were initiated from the shoot meristem (0.5-0.8 mm in length) using the pro-Plant Cell Reports (1999) 18: 424-428

No abstract

No abstract

Pumpkin ( cucurbita maxima )