Phronima sp. is a natural feed with high nutrient content, and it can be an alternative substitute for Artemia. The existence of probiotic bacteria in culture media aims to improve water quality through biodegradation, maintain microbial balance and control pathogenic bacteria, so that nutrients in culture media can be utilized by Phronima sp. This study aimed to determine the effect of fermented organic fertilizer by probiotic bacteria in mass culture media on the population density, biomass production and nutrient content of Phronima sp. and to find the best culture media composition for mass culture of Phronima sp. The study was conducted with a completely randomized design with five treatments and three replications. The treatments were as follows: A: 0% chicken manure, 50% rice bran, 50% tofu waste; B: 25% chicken manure, 37.5% rice bran, 37.5% tofu waste; C: 50% chicken manure, 25% rice bran, 25% tofu waste; D: 75% chicken manure, 12.5% rice bran, 12.5% tofu waste and E: 100% chicken manure. The test animals were Phronima sp., and during cultivation, fertilization was carried out every 3 days. Population density and biomass were observed, and proximate analysis, amino acid profile analysis and fatty acid profile analysis were performed. The highest population density was at treatment B, with a peak population of 98 individuals/L that occurred on the 16th day of maintenance culture, and the highest biomass of 0.51 g was at the same treatment. The highest nutrient content was obtained at treatment B, for which the highest protein proximate analysis value was 58.90%, the proportion of the fatty acids comprised of eicosapentaenoic acid was 7.53%, and lysine amino acids were found at 44.16 ppm.

Daphnia sp. is one kind of natural food that has many advantages. Some advantages of Daphnia sp. is a high nutrient content, according to the size suitable for fish larvae, and the provision of Daphnia sp. in the form of life does not cause a decrease in water quality. Nutrition content on the Daphnia sp. became from the suspended organic and the bacteria which was obtained from the fertilizer that added to the culture media. This research was aimed to found out the effect of fermented quail feces, bread waste, and tofu and determine the best treatment to generate the biomass growth, and the nutrition content of the Daphnia sp.This research used laboratory animals such as Daphnia sp. and a container of concrete tanks as many as four. The methods of this research was used experimental methods with Complete Randomize Design with 4 treatments and population count repetition as 3 times with the density of the Daphnia sp. was 100 ind./l. Treatments of this research were Tretment A (0 % chicken mannure, 50 % tofu waste and 50 % bread waste), B (25 % chicken mannure, 50 % tofu waste and 25 % bread waste), C (25 % chicken mannure, 25 % tofu waste and 50 % bread waste, D (50 % chicken mannure, 25 % tofu waste and 25 % bread waste) with the total amount of the combination was 200 g/l. Data which observed were population density, biomass, and nutrition content. The results showed that the treatment of A was the lowest population density valued 548.67 ind / ml and the highest population density was on C treatment with a density of 1328.67 ind / ml at the peak of the stationary phase. Biomass in treatment C resulted in 336.30 grams and the treatment A yield 82.64 grams, and the content of nutrients with the highest protein is found in the C treatment with a value of 66.80%.Keywords :Daphnia sp.;chicken manure; tofu waste; bread waste; fermentation AbstrakDaphnia sp. merupakan salah satu jenis pakan alami yang memiliki banyak keunggulan.Beberapa keunggulan Daphnia sp.yaitu kandungan nutrisi yang tinggi, ukuranya sesuai dengan bukaan mulut larva ikan, serta pemberian Daphnia sp. dalam bentuk hidup tidak menyebabkan penurunan kualitas air. Kandungan nutrisi dalam tubuh Daphnia sp.berasal dari bahan organik tersuspensi dan bakteri yang diperoleh dari pupuk yang ditambahkan ke dalam media kultur.Penelitian ini bertujuan untukmengetahui pengaruh pemberian kombinasi fermentasi kotoran ayam, roti afkir dan ampas tahu serta mengetahui perlakuan terbaik untuk menghasilkan pertumbuhan, biomass, dan kandungan nutrisi Daphnia sp..Penelitian ini menggunakan hewan uji berupa Daphnia sp. dan wadah berupa bak beton sebanyak empat buah. Metode yang digunakan dalam penelitian ini yaitu dengan metode eksperimental dengan Rancangan Acak Lengkap dengan 4 perlakuan dan pengulangan perhitungan populasi sebanyak 3 kali dengan padat penebaran Daphnia sp. yaitu 100 ind/l. Perlakuan dalam penelitian ini yaitu Perlakuan A (0 % kotoran ayam, 50 % ampas tahu dan 50 % roti afkir), B (25 % kotoran ayam, 50 % ampas tahu dan 25 % roti afkir), C (25 % ...