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Siderophores are small molecular weight (generally 1 kDa) ferric specific ligands produced by variety of organisms to chelate iron under iron limiting conditions. Various assays have been in use to detect and estimate different phenotypes of siderophores. Though there are various methods available for detection of iron specific siderophore or modified method for Cu specific siderophore [chalkophore], reports on modified methods for detection of siderophore having affinity for various other metal ions are scarce. In present study, a modified method was designed for screening siderophores that can bind to heavy metal ions such as Cu 2+

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In present study in vitro phytopathogen suppression activity of siderophoregenic preparations of Ni and Mn resistant Alcaligenes sp. STC1 and Pseudomonas aeruginosa RZS3 SH-94B isolated from soil were found superior over the chemical pesticide. Siderophore rich culture broth and siderophore rich supernatant exerted antifungal activity against Aspergillus niger NCIM 1025, Aspergillus flavus NCIM 650, Fusarium oxysporum NCIM 1281, Alternaria alternata ARI 715, Cercospora arachichola, Metarhizium anisopliae NCIM 1311 and Pseudomonas solanacerum NCIM 5103. Siderophore rich broth and supernatant exhibited potent antifungal activity vis-à-vis oraganophosphorus chemical fungicide; kitazine. The minimum fungicidal concentration required was 25 μl for Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, Cercospora arachichola, Metarhizium anisopliae, Pseudomonas solanacerum and 75 μl for A. alternata.

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