Measurements of the dimensions and membrane rotational frequency of individual erythrocytes steadily tank-treading in a rheoscope are used to deduce the surface shear viscosity of the membrane. The method is based on an integral energy principle which says that the power supplied to the tank-treading cell by the suspending fluid is equal to the rate at which energy is dissipated by viscous action in the membrane and cytoplasm. The integrals involved are formulated with the aid of an idealized mathematical model of the tank-treading red blood cell (RBC) (Keller and Skalak, 1982, J. Fluid Mech., 120:24-27) and evaluated numerically. The outcome is a surface-averaged value of membrane viscosity which is representative of a finite interval of membrane shear rate. The numerical values computed show a clear shear-thinning characteristic as well as a significant augmentation of viscosity with cell age and tend toward agreement with those determined for the rapid phase of shape recovery in micropipettes (Chien, S., K.-L. P. Sung, R. Skalak, S. Usami, and A. Tozeren, 1978, Biophys. J., 24:463-487). The computations also indicate that the rate of energy dissipation in the membrane is always substantially greater than that in the cytoplasm.

Presented is an algorithm for the approximate calculation of the membrane stress distribution and the internal pressure of a steadily tank-treading red cell. The algorithm is based on an idealized ellipsoidal model of the tank-treading cell (Keller, S.R., and R. Skalak, 1982, J. Fluid Mech., 120:27-47) joined with experimental observations of projected length, width, and tank-treading frequency. The results are inexact because the membrane shape and velocity are assumed a priori, rather than being determined via appropriate material constitutive relations for the membrane; these results are, nevertheless, believed to be approximately correct, and show that internal pressure builds up slowly as cell elongation increases, rising more rapidly as the deformed cell approaches the limiting geometry of a prolate ellipsoid. The maximum shear stress resultant in the membrane was found to be below but approaching the yield point range at the highest shear rate applied.

This paper treats the steady flow fields generated inside and outside an initially circular, inextensible, cylindrical membrane filled with an incompressible viscous fluid when the membrane is placed in a two-dimensional shear flow of another viscous fluid. The Reynolds numbers of both the interior and exterior flows were assumed to be zero (‘creeping flow’), but no further approximations were made in the formulation. A series solution of the resulting free boundary-value problem in powers of a dimensionless shear rate parameter was constructed through fifth order. When combined with a conformal coordinate transformation this series gave accurate results for large deformations of the membrane (up to an aspect ratio of 2.5). The rather tedious algebraic manipulations required to obtain the series solution were done by computer with a symbol-manipulation program (reduce), which both formulated the boundary-value problems for each successive order and solved them. Results are presented which show how the shear rate and fluid viscosities influence the internal and external velocity and pressure fields, the membrane deformation and its ‘tank-treading’ frequency, and the membrane tension.This work demonstrates that classical perturbation techniques combined with computer algebra offer a useful alternative to purely numerical methods for problems of this type.

The nonlinear problem of the steady-state interaction of a closed fluid-filled cylindrical elastic membrane with a slow viscous shear flow has been solved by a series-expansion technique. The problems of successive orders were both formulated and solved by a symbolic manipulation program, and the calculations were carried to sixth order in a dimensionless parameter related to the applied shear rate. Moderately large deformations (aspect ratios approaching 3) fall within the range of this analysis, which yields the dependences of the following global variables on the system parameters: membrane deformation, orientation, and strain, as well as tank-treading frequency, and mean internal pressure. The solution for the flow field around an isolated capsule is also used to calculate the apparent viscosity of a dilute suspension of flexible cylindrical particles, which yields the paradoxical result that the apparent viscosity decreases as the internal viscosity increases.

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