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Computational simulation for the fluid flow in a paper based microfluidic system was performed and was experimentally validated in this work. Comsol Multiphysics based simulation was performed and the module used was species transport in porous medium. Two separate simulation for the reagent distribution and the interaction of sample with the immobilized reagent in paper based microfluidics devices were carried out. This was performed by varying the parameters such as adsorption constant, diffusivity and average velocity of fluid in the porous medium for finding the concentration profile. Finer mesh were used for the simulation which gives more accurate results with less computational time. The reagent distribution was experimentally validated by dropping methylorange indicator over Whatman filter paper.

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