The rough and pitted surfaces seen on acrylic dentures act as niduses for biofilm formation and colonization of microorganisms. Denture cleansers are the most preferred for chemical cleansing, which has been suggested for the disinfection of prostheses. The aim of the study was to evaluate four different denture cleansers on tea stain removal from heat cure clear acrylic resins (DPI heat cure) material. Forty clean acrylic specimens of dimensions 40 mm 3 20 mm 3 1 mm were fabricated. The samples were then placed in fresh human unstimulated saliva for 2 min to form an initial pellicle layer and facilitate the uptake of stain, and they were washed with water. The samples were divided into 4 batches of 10 each. One-way ANOVA, Tukey' HSD, and Students' paired t-test were employed to identify the significant groups at 5% level. The mean value in Batch C, i.e., using Clinsodent powder (0.62 6 0.32), was significantly higher than the mean values in Batch A, i.e., using Corega Bio Tabs (0.34 6 0.14), Batch B, i.e., using fitty dent tabs (0.19 6 0.19), and Batch D, i.e., using Steradent powder (0.25 6 0.11) (p  0.05). This study revealed that all the four immersion denture cleansers were effective in removing the tea stains from heat cure acrylic resins. Among the four cleansers, Clinsodent powder removed the tea stains to the greatest extent.