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Ipomoea biloba is a plant, which belongs to Convolvulanceae family and also has more medicinal values. It is an aquatic creep runner plant used as a medical herb for various diseases such as asthma and rheumatism and dried leaves are used to apply for burns. In the current investigation, the Methanol extract of Ipomoea biloba leaves was experimented to evaluate the phytochemical properties, in vitro antioxidant activity and in vitro anticancer assay on MCF7 cell line. Plant components can be used to extract both hydrophilic and lipophilic compounds. Methanol is an excellent solvent of its polarity. Because methanol is highly volatile, we can remove the solvent by distillation at a low temperature after extraction. The leaves of Ipomoea biloba extract used in an analysis which shows the ability to produced estrogen comes froth as estradiol via estrogen receptor in the cytoplasm of the cell. Phytochemical screening showed the presence of carbohydrate, amino acids, alkaloids, saponin, steroids, terpenoids and phenols. The antioxidant activity of Methanol extract indicates the significant antioxidant content it and against with standard ascorbic acid. The results of this analysis highlight the interest of Ipomoea biloba extract for the isolation of anticancer molecules.