When blood cannot flow to the brain, a stroke occurs. A blockage or burst blood vessel inside the brain is a common culprit for this disturbance in blood flow because they both limit oxygen to the brain tissue. If this happens, the brain's oxygen-starved cells begin to die quickly, so it's important to get medical attention at once for the patient's chances of recovery. The major objective of this study is to investigate potent thrombolytics from natural sources that are used to treat stroke. Natural sources have been found to exhibit thrombolytic action, with active molecules also extracted and described. The ligand strength was confirmed in the present work using the binding energy, which is based on computer-aided molecular modeling. Vanillylamine exhibited a stronger docking score and had greater thrombolytic potency than other drugs. The main objective of this study is to find an effective treatment for heart attacks. The protein and ligand combination had an affinity of -5.40 kcal/mol, which further suggests that it may be employed in future studies to create a potential inhibitor against stroke.