Objective: Uric acid is an end product of purine metabolism and it has two different functions such as pro-oxidant and anti-oxidant. Where, pro-oxidant and anti-oxidants are opposite in action. Oxidative stress and endothelial dysfunction are a foremost cause of complications in diabetes mellitus, where uric acid may play a major role in this process. Hence, the present study has been designed to evaluate antioxidant activity of uric acid and its correlation with oxidative stress and endothelial dysfunction in type 2 diabetic subjects. Methods: We included 120 subjects in this study with age group of 39 -60 years. Among these 60 were type 2 diabetic subjects and 60 were healthy controls. The estimation of biochemical parameters such as blood sugar, lipid profile, uric acid, and homocysteine are measured in fully auto-analyzer with well recognized methods. MDA measured by TBARS method, total antioxidant capacity as FRAP and NO estimated by Kinetic cadmium method in spectrophotometer. Results: The study was found significant elevation of triglyceride, LDL and MDA and significant lower level of FRAP and NO in T2DM than healthy control. Uric acid was insignificant in T2DM compared to healthy control. However, uric acid has significant correlation with FRAP (r=0.2116, p=0.02) and moderate correlation with triglyceride (r=0.1736, p=0.0579) and homocysteine (r=0.1779, p=0.0519). MDA was negatively and NO was positively correlated with uric acid but statistically insignificant. Conclusion: We have found antioxidant activity of uric acid where it was determined by significant positive correlation with FRAP in type 2 diabetes mellitus.

Introduction: Endothelial dysfunction (ED) is a well known initial stage of atherosclerosis and cardiovascular disease (CVD) in general population. Recently studies shown increased HbA1c is a marker for cardiovascular risk in subjects with diabetes and without diabetes. Atherogenic index of plasma (AIP) is another risk factor for cardiovascular events. Hence, the study has been designed to assess the correlation of HbA1c with AIP and ED in type-2 diabetic subjects to forecast risk of cardiovascular events. Materials and Methods: Hundred subjects are enrolled into study with the age of 38 to 58 years, among these 50 were type 2 diabetic subjects and 50 were healthy controls. HbA1c was estimated by immuno-turbidimetric method. Nitric oxide (NO) was measured by kinetic cadmium reduction method and AIP was calculated by standard formula Log (triglyceride/HDL-c). 'Kruskal Wallis' test was used to perform statistical variables between the study groups. The association between the variables was executed by "Pearson correlation test". Result: HbA1c level was shown to be significantly higher in type-2 diabetic subjects than healthy controls. Significant lower level of NO was observed in type-2 diabetic subjects than healthy controls. AIP level was significantly higher in type-2 diabetic subjects than healthy controls. The present study also observed significant correlation of HbA1c with NO and AIP. Conclusion:We have found that HbA1c has significant correlation with NO and AIP. Therefore, estimation of HbA1c may be a predictable marker for cardiovascular events in type-2 diabetic subjects.