Background: Prevention and control of HIV/AIDS depends on general knowledge and attitude towards HIV/AIDS. So the present study was conducted with the aim to assess knowledge, attitude, and preventive practices regarding HIV infection and AIDS among rural peoples.Methods: A cross sectional study was conducted among peoples age more than 15 years in a villages which comes under field practice area of rural health training centre (RHTC) of our medical college. Total 396 individuals were interviewed during March 2015 to April 2015 by using predesigned and pretested questionnaire after taking written informed consent.Results: 71.5% knows uninfected faithful partner and 82.1% thinks use of condom will prevent transmission of HIV/AIDS to their partners. 46.7% thinks mosquito bite, 37.6% thinks sharing food transmit HIV/AIDS. 83.1% knows pregnancy and 79.3% knows breastfeeding transmit HIV to their children. 61.1% willing for not to keep HIV status secret, 81.6% willing to take care of relatives having HIV, 88.6% allow HIV positive teachers to continue teaching and 68.4% will buy vegetables from HIV positive shopkeeper. 36.8% knows nearby place where condoms are available and 49.7% knows nearby place for HIV testing.Conclusions:People have good knowledge about HIV transmission i.e. faithful partner, condom use, pregnancy and breast feeding. Still some misconceptions need to be address regarding mosquito bite and sharing food with person having HIV/AIDS. Participants have poor knowledge about nearby availability of services related to HIV/AIDS.