Objective-The Extended Spectrum Beta Lactamases (ESBL) producing bacteria are increasingly causing urinary tract infection (UTI) both in hospitalized and outpatients. Failure to identify them also contributes to their uncontrolled spread. Therefore, identification of the resistant phenotypes is important, particularly in developing countries where excessive use of antibiotics and lack of adequate antimicrobial resistance surveillance is there. The present study was conducted with an objective to examine the incidence of ESBL producing stain of Klebsiella pneumoniae and E. coli from UTI. Materials and methods-A total of 200 urine samples were analyzed during the period of study. Isolates of E. coli and Klebsiella pneumoniae were selected for further study. These isolates were tested for susceptibility to antibiotics like cephotaxime, chloramphenicol, ciprofloxacin, gentamycin and amoxicillin. ESBL production was tested by double disk synergy test recommended by Clinical and Laboratory Standards Institute. Result-Out of 200 urine samples, 77 and 58 isolates were E.coli and Klebsiella pneumoniae respectively. Among these uropathogens, 45.45% of E. coli and 31 % of Klebsiella pneumoniae were ESBL positive. ESBL producing strains were also found to be resistant to other antibiotics tested. Conclusion-The investigation has demonstrated that it is important for clinical microbiology laboratories to have the ability to detect a report on ESBL production in uropathogens of Gram-negative bacteria particularly with Klebsiella pneumoniae and E.coli isolated from urinary tract infection.