Evidence suggests that postural changes in systemic blood pressure may significantly affect blood flow in the dental pulp. This in vivo study examined the responses of pulpal perfusion, systemic blood pressure, and heart rate to postural changes in humans. The experiments were done on 21 premolars in 16 participants aged 20-31 yrs. Pulpal blood flow recordings were measured by means of a laser Doppler Flowmeter. A blood pressure monitor was used to record blood pressure and heart rate. All measurements were simultaneously recorded for 1 min, 5 min after participants made postural changes. Changing from supine to standing caused a significant reduction in pulpal perfusion, while heart rate and diastolic blood pressure increased significantly. A significant non-linear relationship was found between percentage changes in pulpal perfusion and heart rate resulting from standing up. We speculate that when patients arise from the supine position, the shift in venous blood to the legs transiently (2-10 sec) lowers venous return and cardiac output, causing less inhibition of the vasomotor center, which, in turn, results in increased heart rate and blood pressure, but a decrease in pulpal blood flow. These results suggest that pulpal blood flow is affected by postural change, presumably via the autonomic nervous system.