We examined the ability of recombinant adeno-associated no detectable constitutive expression of luciferase and that virus (rAAV) to transfer regulated gene expression into T luciferase gene expression remained inducible for at least cell lines. An AAV-based vector containing the neomycin 180 days. Luciferase expression was activated by PMA resistance gene and expressing the firefly luciferase (luc) and ionomycin and by anti-CD3 antibodies and was gene under the regulatory control of the interleukin 2 proinhibited by cyclosporin A. Examination of G418-resistant moter (pAAV-luc) was generated and adenovirus-free clones showed that rAAV-luc had integrated into the host rAAV (rAAV-luc) was produced from this vector. Transfecchromosomes but that some of the clones lost some of tion of pAAV-luc into the human T cell line Jurkat resulted the transferred DNA or lost expression from the transferred in luciferase expression while infection of Jurkat T cells DNA. These results indicate that rAAV can transfer and with rAAV-luc resulted in significant luciferase expression integrate regulated gene expression into T cell lines but only after selection for neomycin-resistant cells. Long-term that the transferred genetic material may be lost or its growth of transduced Jurkat T cells showed that there was expression may be silenced over time.