The stilbenoid pathway is responsible for the production of resveratrol in grapevine (Vitis vinifera L.). A few transcription factors (TFs) have been identified as regulators of this pathway but the extent of this control has not been deeply studied. Here we show how DNA affinity purification sequencing (DAP-Seq) allows for the genome-wide TF-binding site interrogation in grape. We obtained 5190 and 4443 binding events assigned to 4041 and 3626 genes for MYB14 and MYB15, respectively (approximately 40% of peaks located within À10 kb of transcription start sites). DAP-Seq of MYB14/MYB15 was combined with aggregate gene co-expression networks (GCNs) built from more than 1400 transcriptomic datasets from leaves, fruits, and flowers to narrow down bound genes to a set of high confidence targets. The analysis of MYB14, MYB15, and MYB13, a third uncharacterized member of Subgroup 2 (S2), showed that in addition to the few previously known stilbene synthase (STS) targets, these regulators bind to 30 of 47 STS family genes. Moreover, all three MYBs bind to several PAL, C4H, and 4CL genes, in addition to shikimate pathway genes, the WRKY03 stilbenoid co-regulator and resveratrol-modifying gene candidates among which ROMT2-3 were validated enzymatically. A high proportion of DAP-Seq bound genes were induced in the activated transcriptomes of transient MYB15-overexpressing grapevine leaves, validating our methodological approach for delimiting TF targets. Overall, Subgroup 2 R2R3-MYBs appear to play a key role in binding and directly regulating several primary and secondary metabolic steps leading to an increased flux towards stilbenoid production. The integration of DAP-Seq and reciprocal GCNs offers a rapid framework for gene function characterization using genome-wide approaches in the context of non-model plant species and stands up as a valid first approach for identifying gene regulatory networks of specialized metabolism.

Computer programming is generally understood to be highly challenging and since its inception a wide range of approaches, tools and methodologies have been developed to assist in managing its complexity. Relatively recently the potential benefits of collaborative software development have been formalised in the practice of pair programming. Here we attempt to 'unpick' the pair programming process through the analysis of verbalisations from a number of commercial studies. We focus particularly on the roles of the two programmers and what their key characteristics and behaviours might be. In particular, we dispute two existing claims: (i) That the programmer who is not currently typing in code ("the navigator") is constantly reviewing what is typed and highlighting any errors (i.e. acting as a reviewer); (ii) That the navigator focuses on a different level of abstraction as a way of ensuring coverage at all necessary levels (i.e. acting as a foreman). We provide an alternative model for these roles ("the tag team") in which the driver and navigator play much more equal roles. We also suggest that a key factor in the success of pair programming may be the associated increase in talk at an intermediate level of abstraction.

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