Algae are aquatic organisms that can be found in a wide range of water bodies. Algae, a form of aquatic organism, is found in many different water types. Besides being relatively easy to maintain, algae are also numerous, making them a good choice for biomass production. The filamentous Spirogyra sp., a common green alga, tends to grow in freshwater. It is said that this macroalga has a wide variety of biotechnological applications. Research in this area highlights biomass's creation and builds on our understanding of the composition of macroalgae generated in fish farm wastewater. A study of Spirogyra in undisturbed fish farm wastewater was conducted in this study. Various algal species were evaluated for their qualities, including biomass yields and productivity, protein, fat, and carbohydrates. This investigation has confirmed that the nutrients in fish farm effluent are suitable for cultivating algal biomass. Protein, lipid, and carbohydrate levels in unaltered fish farm effluent were the highest for Spirogyra, with percentages of 19.03, 8.38, and 45.71%, respectively. Thus, it was the most suitable organism for various biomass-based applications and nutrient removal.

Due to high demand in the industry field, fuel supply has been reduced due to overconsumption. Algae served as the most promising biofuels; Cyanobacteria is preferred as a material for producing biodiesel based on the comparative advantage. Cyanobacteria can produce high lipids profiles that can be used to produce biofuel or biodiesel. The present investigation aimed to identify the application of Cyanobacteria that have proposed lipid standards for the algae biofuel industry. Three main objectives are involved in these experiments: to isolate and identify different strains of Cyanobacteria, convert the lipid from microalgae into biodiesel through transesterification, and estimate the proposed lipid standard of Cyanobacteria for the algae biofuel industry. The microalgae involve isolated using a plankton net. The water sample is brought into the lab for the identification of Cyanobacteria. After the Cyanobacteria are identified, the Cyanobacteria undergo mass cultivation to ensure the biomass is enough for lipid screening. After lipid content is identified, the lipid in the Cyanobacteria is extracted and further transesterification process. The GC analysis showed the variation of fatty acid in this cyanobacterium, a saturated, monounsaturated, and polyunsaturated fatty acid. The study also revealed that hexadecanoic acid, pentadecanoic acid and pentadecanoic acid was found in Oscillatoria sp. Lipid screening can determine the quantity of lipid present in the Cyanobacteria to estimate the lipid content for biofuel production.