PEGylated PPI dendritic scaffold was used for the delivery of an anti-leukemic drug, Prednisolone. The current study evolves and emerges the use PEGylated poly dendritic scaffold for the delivery of this drug. In this the prednisolone was loaded and synthesized with PEGylated PPI dendritic scaffold. Estimated the biodistribution of prednisolone in different vital organ was carriedout by HPLC method and the study was promising the distribution of drug candidate.