Background: In present work attempt has been made to develop and validate a simple and sensitive UV spectrophotometric method for simultaneous estimation of Telmisartan and Metformin Hydrochloride. Objectives: To develop simple, precise, robust, sensitive and accurate UV-Spectrophotometric method for the simultaneous estimation of Telmisartan and Metformin HCl and to formulate the combined tablet dosage formulation of Telmisartan and Metformin HCl respectively. Methods: The optimum condition for the analysis of the drug was established with Methanol as solvent. Maximum absorption wavelength were found to be 296nm and 237nm for Telmisartan and Metformin Hydrochloride. It showed linear response between the concentration ranges of 2-12µg/ml and 0.1-0.6 µg/ml for Telmisartan and Metformin Hydrochloide espectively. The linear regression coefficient was found to be 0.999. The method was validated for linearity, Precision, ruggedness, specificity, Sensitivity as per ICH guidelines and all the values of validation was found to be within the acceptance. Hence it can be concluded that method was new, simple, selective, specific, precise for simultaneous estimation of Telmisartan and Metformin Hydrochloride in bulk powder.

Background:The combination of Ciprofloxacin and Ornidazole is used for the effective treatment of intra-abdominal infections. Many branded and generic combined dosage formulations of Ciprofloxacin and Ornidazole are available in market. Objective: To develop and validate rapid, sensitive, simple and accurate reverse phase ultra-fast liquid chromatographic method for the determination of Ciprofloxacin and Ornidazole in combined dosage forms. Methods: The separation was carried out by using Phenomenex Luna C 18 (250×4.6 mm, 5 µm) column as stationary phase and acetonitrile: methanol: 0.1% formic acid as a mobile phase in ratio of 35:35:30 % v/v/v. The analysis was performed at flow rate of 0.8 ml/min using PDA detector at 300 nm. The column temperature was maintained at 20°C for better separation and resolution. Results: The retention time for Ciprofloxacin HCl and Ornidazole was found to be 2.1 min and 4.2 min respectively. The developed method was validated with respect to specificity, linearity and range, precision, robustness and accuracy according to ICH guidelines. The method was found to be linear between the concentration ranges of 2-10 µg/ml with correlation coefficient of 0.999. The % relative standard deviation of precision and robustness was found to be less than 2%. The percent accuracy was found to be 98-103% and 99-102% for Ciprofloxacin HCl and Ornidazole respectively. Conclusion: The method is applicable for the routine analysis and quality control of Ciprofloxacin HCl and Ornidazole in combination.