Materials and methods
ReagentsFolin-Ciocalteau reagent, gallic acid, quercetin, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,4,6-tripyridyl-1,3,5-triazine (TPTZ), 2,2-Azinobis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), standard nonadecanoic acid methyl ester (19:0) and trans-resveratrol internal standard were from Sigma-Aldrich. All other reagents were of analytical grade.
SamplesUnfermented Bordo seeds were obtained from an industry in the city of Toledo, Paraná State, Brazil, before fermentation process. Fermented seeds were obtained at a winery located in Quatro Pontes municipality, Paraná State, Brazil, after fermentation process. The seeds were separated from the peels and peduncles, washed, sun-dried, sieved, packed in vacuum, and stored at room temperature.