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A micro-scanning technique of SXRF (Synchrotron Radiation X-ray fluorescence) combined with multivariate statistical analysis was employed. The elements positional distribution in the kidney tissues of tree groups of Wistar rats (1, normal; 2, treated by CdCl 2; 3, Cd exposed rats treated with new complex) were obtained. The results show that Cd mainly distribute in the renal cortex of rat treated by CdCl 2. The level of Cd in the renal medulla is very low or not detectable. The elements measured in renal cortex of normal rat can be classified into two groups, Cu, Zn, Mn and Se belong to the same group. In acute CdCl 2 poisoning, Cd and Se have a very strong correlation, and the classification is different from normal renal cortex. After administration of a new chelator, Zn and Se have a very strong co-location and correlation, the classification of elements tend to return to normal distribution.

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