Monoclonal antibodies with specific antigens have been widely used as targeted therapy for cancer. Hep88 mAb is a monoclonal antibody which shows specific binding with anti-cancer effects against the HepG2 cell line. However, its mechanisms of action are still not completely understood. We examined cell cycling and apoptosis by flow cytometry and mRNA expression of factors involved in apoptosis and paraptosis in Hep88 mAb-treated HepG2 cells by real-time PCR. The cell-cycle analysis demonstrated that growth-inhibitory activity was associated with G2/M cell cycle arrest. Hep88 mAb induced a significant increase in apoptotic cell populations in a dose-and time-dependent manner. The mRNA expression results also suggested that the process triggered by Hep88 mAb involved up-regulation of tumor suppressor p53, pro-apoptotic Bax, Cathepsin B, Caspase-3 and Caspase-9, with a decrease of anti-apoptotic Bcl-2 -thus confirming paraptosis and apoptosis programmed cell death. These findings represent new insights into the molecular mechanisms underlying the anti-cancer properties of Hep88 mAb in liver cancer cells.

Moodle is applied as an online learning management system in the Faculty of Medicine, Thammasat University (TU), where the curriculum has been based on problem-based learning (PBL) since 2015. Little research on the effectiveness of Moodle and students’ attitudes during their studies at the pre-clinical medical level within a PBL curriculum has been conducted. Hence, this cross-sectional study focuses on second- and the third-year (MD-2 and MD-3) preclinical medical students’ attitudes towards Moodle and its effectiveness in the PBL curriculum by using a 27-itemed self-constructed online questionnaire. Additionally, students’ preferences as regards the materials managed by the Moodle platform were also evaluated as students’ preferred behaving style (SPBS). After obtaining ethical approval from the Human Ethics Committee of TU No. 1 (Faculty of Medicine), the data was collected and analyzed by medians and inter-quartile ranges, Mann-Whitney U test, and Spearman correlation (ρ). After analyzing the data, the results indicated the following: (1) the top four attitudes scores, (2) the two lowest-attitude scores, (3) the top five effectiveness scores (Ef), (4) the three lowest effectiveness scores, and (5) the top three SPBS scores. Interestingly, a stronger positive Spearman’s correlation (ρ) between ‘Prefer doing quizzes in Moodle to in classroom’ and ‘Moodle enhances learning capability’ was found for MD-3 compared with MD-2. This study reports that Moodle is preferable as a platform supporting students’ self-learning within a PBL curriculum. However, to use the Moodle platform to the highest benefit, instructors need to upgrade its capabilities, especially in the area of student-teacher interaction and the enhancement of students’ critical thinking and creativity, as demonstrated by the lowest scores obtained in this study.

BackgroundPhytochemical products have a critical role in the drug discovery process. This promising possibility, however, necessitates the need to confirm their scientific verification before use. Hence, this study aims to evaluate (1) the antioxidant activity, (2) cytotoxicity potential, and (3) the effect on ultrastructural alteration in gastric cancer cell lines through exposure to fractions of three local Northeastern Thai edible plants.MethodsPlants, Syzygium gratum, Justicia gangetica and Limnocharis flava were extracted with ethyl acetate, and each crude extract analysed for their total phenolics content by Folin-Ciocalteu method. Their antioxidant activity was assessed using the ABTS system. The extracts were then assayed for cytotoxicity on two gastric cancer cell lines Kato-III and NUGC-4, and compared with Hs27 fibroblasts as a control using the MTT assay. The cell viability (%), IC50 values, as well as the ultrastructural alterations were evaluated after treatment with one way analysis of variance (ANOVA).ResultsThe total phenolic values of the ethyl acetate extracts were well correlated with the antioxidant capacity, with extracted product of S. gratum displaying the highest level of antioxidant activity (a 10-fold greater response) over J. gangetica and L. flava respectively. Exposure of S. gratum and J. gangetica extracts to normal cell lines (Hs27) resulted in marginal cytotoxicity effects. However, through a dose-dependent assay S. gratum and J. gangetica extracts produced cytotoxicological effects in just over 75 percent of Kato-III and NUGC-4 cell lines. In addition, apoptotic characteristic was shown under TEM in both cancer cell lines with these two extracts, whereas characteristics of autophagy was found in cell lines after post exposure to extracts from L. flava.ConclusionsFrom these three plants, S. gratum had the highest contents of phenolic compounds and antioxidant capacity. All of them found to contain compound(s) with cytotoxicity in vitro on cancer cells but not on normal cell lines as resolved in tissue culture and ultrastructural analysis. This is the first report to show the effect on cellular alteration as apoptosis of an ethyl acetate extract of S. gratum and J. gangetica. Further studies are now focused on individual isolates and their function, prioritizing on S. gratum and J. gangetica for the development of novel therapeutics and combatants against cancer.

Coronary heart disease (CHD) is the leading cause of death worldwide. Dietary inclusion of monounsaturated fatty acids (MUFA) such as olive oil (OO) can reduce CHD risk. Tea seed oil (TSO) from Camellia oleifera grown in Thailand, with a MUFA content similar to OO, may be an alternative to OO when a higher cooking smoke point is desired. The lipid profiles, liver histology, and serum chemistries of hamsters fed high‐fat diets (TSO, OO, grape seed oil or butter; 14% by weight) are analyzed. After three weeks of feeding, TSO and OO groups have similar plasma low‐density lipoprotein cholesterol (LDL‐C), very‐low‐density lipoprotein cholesterol (VLDL‐C), triacylglycerols (TAG), high‐density lipoprotein cholesterol (HDL‐C), and total cholesterol (TC) levels. Moreover, TC/HDL‐C and LDL‐C/HDL‐ C ratios are also comparable. Similar to the OO group, the TSO group has significantly lower plasma LDL‐C, VLDL‐C, TAG, TC, and a lower TC/ HDL‐C ratio as compared to the butter group. Some minor liver pathological lesions commonly found in rodents fed high‐fat diets are observed. Thai TSO may be a healthy option for cooking, and clinical studies are warranted. Practical Applications: With its high smoking point, TSO is attractive to Asian consumers for their styles of cuisine. As it is produced locally, its price is cheaper than imported OO. This study demonstrates TSO's health benefits in lowering lipid profiles similar to OO. Based on this fact, this study supports Thai TSO cultivation in northern Thailand as a means of increasing rural villagers’ income. Hamsters are divided into five groups: tea seed oil, olive oil, grape seed oil, low fat, and butter. Tea seed oil favorably lowers plasma low‐density lipoprotein cholesterol (LDL‐C) in hamsters fed high‐fat diets.

Hepatocellular carcinoma (HCC) is a leading cause of cancer death worldwide. Presently, targeted therapy via monoclonal antibodies to specific tumor-associated antigens is being continuously developed. Hep88 mAb has proven to exert tumoricidal effects on the HepG2 cell via a paraptosis-like morphology. To verify the pathway, we then demonstrated downstream up-regulation of caspase-3, caspase-8 and caspase-9, assessingmRNA expression by real-time PCR and associated enzyme activity by colorimetric assay. Active caspase-3 determination was also accomplished by flow cytometry. Active caspase-3 expression was increased by Hep88 mAb treatment in a dose-and time-dependent manner. All of the results indicated that Hep88 mAb induced programmed cell death in the HepG2 cell line from paraptosis-like to apoptosis by downstream induction of caspases. These conclusions imply that Hep88mAb might be a promising tool for the effective treatment of HCC in the future.