In India testing for Human Immunodeficiency Virus, Hepatitis-B Virus, Hepatitis-C Virus and Syphilis is mandatory for all blood donors. Transmission of HIV, HBV, HCV and Syphilis through blood and blood products can be reduced to a great extent by efficient and reliable screening of the blood to be transfused. This study was aimed to find out the seroprevalence of the transfusion transmitted diseases. OBJECTIVES: The present study was conducted to estimate the prevalence of transfusion transmitted infections in voluntary blood donors in Victoria hospital blood bank in Bangalore in India. MATERIALS AND METHODS: A retrospective study was conducted with 3910 voluntary blood donors of which 3505 donors were from voluntary blood donation camps and 405 voluntary blood donors donated in blood bank. All donors met the standard inclusion and exclusion criteria followed in India. 1 The donors had donated blood to Victoria hospital, Bangalore from 1 st January 2013 to 31 st December 2013. All voluntary donor's blood sample were tested for HIV,HBV,HCV and Syphilis by HIV-ELISA (Enzyme Linked Immuno-Sorbent Assay), HBSAg-ELISA,HCV-ELISA and Venereal Disease Research Laboratory tests respectively by standard procedures. RESULTS: Out of the total 3910 blood samples tested only 1 donor(0.025%) was HIV positive, 28 donors (0.7%) were HBSAg reactive, none of the samples were HCV reactive, 1 donor (0.025%) was VDRL reactive. CONCLUSION: Though prevalence of HIV, HBV, HCV and Syphilis have reduced over the years. Blood is still one of the main sources of transmission of infections. HIV, hepatitis B, hepatitis C viruses and syphilis are prevalent even among voluntary blood donors.