Platelet-poor plasma (PPP) can be obtained by various centrifugation techniques. This study aims to analyze platelet count in PPP produced by three different centrifugation techniques. Samples came from a healthy adult who underwent medical check-up and had been given informed consent. A total of 2.7 mL of blood samples were collected using three citrated tubes. Samples were centrifuged by three different techniques to obtain PPP: 1,500 g for 15 minutes twice, 3,000 g for 15 minutes, and 3,260 g for 10 minutes. The platelet count of each PPP was examined using a hematology analyzer. All three centrifugation techniques produced <10,000/µL platelets in all PPP from 31 samples. The twice centrifugation of 1,500 g for 15 minutes produced a median number of platelets which was 1×10 3 /µL (0-3). The 3,000 g centrifugation for 15 minutes produced a median number of platelets which was 1×10 3 /µL (0-5). The 3,260 g centrifugation for 10 minutes produced a median number of platelets which was 2×10 3 /µL (0-5). A comparison of platelet count showed a significant difference (p<0.05) among the three centrifugation techniques. The three centrifugation techniques in this study were able to produce PPP. The centrifugation technique of 1,500 g for 15 minutes twice produced the lowest number of platelets.

This was observational research with cross sectional type of study conducted from January to October 2020 at Dr. Soetomo General Hospital, Surabaya. The subjects of the research were 30 children’s patients with febrile neutropenic episodes with malignancy based on the criteria of the American Society of Clinical Oncology and Infectious Disease Society of America, which were grouped into 2 groups based on blood culture results from secondary data. The control group was hemophilia patients without any complaint. The presepsin level was checked using ELISA. The differences in presepsin levels in positive and negative blood cultures and the control were analyzed using T2 free samples or Mann-Whitney U according to the data distribution. Median (min-max) presepsin levels in positive, negative and control cultures: 4.1(0.3 - 14) ng/mL, 2.95(0.2 - 13.8) ng/mL, 0.1(0.1 - 0.5)ng/mL. There is no significant difference in presepsin levels between positive and negative blood culture (p = 0.606). There is a significant difference between positive and negative culture presepsin levels towards controls (p = 0.001 and p = <0.001). There is an increase in the level of presepsin in febrile neutropenic pediatric patients with malignancy, but this examination cannot differentiate between bacteremia and non-bacteremia infections.