Research the imaging detection technology of tiny fiber optic endoscope based on EMCCD low-light camera for narrow lumen and low-light imaging, build a test system and conduct performance test analysis. A bimodal endoscopic imaging detection system was set up with a colour camera and a low-light EMCCD camera. The method of combining visual cognition with the definition evaluation function based on discrete cosine transform, is employed to evaluate the performance of optical fiber endoscope detection system based on EMCCD camera. Then use EMCCD low-light camera to perform weak fluorescence imaging test of pig stomach tissue . The test results show that under the given experimental conditions, the minimum light intensity is 230 Lux required for the colour camera system, while the minimum light intensity is 40 Lux required for the EMCCD camera system, and the weak fluorescence signal can be well excited and displayed under low light conditions. Based on the low-light imaging performance of EMCCD, it can be used for better endoscope miniaturization.

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