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Abstractstrain BUP2 (MTCC No. 5924), a novel bacterium isolated from the rumen of the Malabari goat was explored in this study for its efficiency in the production of lipase in Benjamin Unni Priji medium supplemented with 1% groundnut oil. Plackett-Burman and Box-Behnken designs were applied for optimizing the culture parameters statistically for the enhanced production of lipase; and temperature (28°C), pH (6) and incubation time (24 h) were found as significant factors for increasing the production of lipase by 11% (from 152 to 171 U/mL). Using (NH

Solid-state fermentation (SSF) holds tremendous potentials for the production of industrially significant enzymes. The present study describes the production of lipase by a novel rumen bacterium, Pseudomonas sp. strain BUP6 on agro-industrial residues. Pseudomonas sp. strain BUP6 showed higher lipase production when grown in Basal salt medium (BSM) supplemented with oil cakes. Initially, five different oil cakes (obtained after extracting oil from coconut, groundnut, cotton seed, gingelly or soybean) were screened to find out the most suitable substrate-cum-inducer for the production of lipase. Among them, groundnut cake supported the maximum production of lipase (107.44 U/gds). Box-Behnken Design (BBD), followed by response surface methodology (RSM) was employed to optimize the culture parameters for maximizing the production of lipase. Using the software Minitab 14, four different parameters like temperature, pH, moisture content and incubation time were selected for the statistical optimization, which resulted in 0.7 fold increase (i.e., 180.75 U/gds) in production of lipase under the optimum culture conditions (temperature 28˚C, pH 5.9, moisture 33% and incubation 2 d). Thus, this study signifies the importance of SSF for the production of industrially-significant lipase using agro-industrial residues as solid support.* Corresponding author. P. A. Faisal et al.126

Owing to wide spectrum of catalytic reactions both in aqueous and non-aqueous media, microbial lipases occupy an unquestionable position among the biocatalysts. The chemo-, regio- and enantio-specificities of lipases have contributed to their versatile applications in biotechnology. As far as global scenario is concerned, microbial lipases - especially from bacteria and fungi - contribute to the choice of interest to meet the commercial needs. At this context, this review critically looks into the major domains of microbial lipases with an industrial perspective, which include: properties, secretion and industrial applications with appropriate illustrations. Due to great specificity and versatility of the reactions catalyzed, lipases claim unique applications in various process and products industries engaged in food, dairy, fats and oils, detergency, tannery, pharmaceutics and cosmetics.

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