In the present work, the wet-chemical precipitation technique is employed to prepare Zinc/Magnesium doped hydroxyapatite (HAP). In doped HAP, the X-ray diffraction peak shifts to a higher angle because of the contraction of the lattice parameters along a - axis. The Raman peaks at 519, 440, 1464 cm-1 indicate the presence of Mg, Zn and CO32- in doped HAP respectively. The Field Emission Scanning Electron Microscopy (FESEM) measures the grain size of pure, 5% Zn and 5% Mg doped HAP, as 275, 510, and 251 nm respectively. Transmission Electron Microscopy (TEM) confirmed the morphological change in HAP. The X-ray photoelectron spectroscopy (XPS) identifies the presence of Mg2+ and Zn2+ in doped HAP. The dopant elevates the hardness and dielectric constant, so the strength and the bone growth of HAP increases. All the doped samples show excellent antibacterial, antifungal and antibiofilm activities than the pure HAP.