Purple foliage always appears in Camellia sinensis families; however, the transcriptional regulation of anthocyanin biosynthesis is unknown. The tea bud sport cultivar ‘Zijuan’ confers an abnormal pattern of anthocyanin accumulation, resulting in a mutant phenotype that has a striking purple color in young foliage and in the stem. In this study, we aimed to unravel the underlying molecular mechanism of anthocyanin biosynthetic regulation in C. sinensis. Our results revealed that activation of the R2R3-MYB transcription factor (TF) anthocyanin1 (CsAN1) specifically upregulated the bHLH TF CsGL3 and anthocyanin late biosynthetic genes (LBGs) to confer ectopic accumulation of pigment in purple tea. We found CsAN1 interacts with bHLH TFs (CsGL3 and CsEGL3) and recruits a WD-repeat protein CsTTG1 to form the MYB-bHLH-WDR (MBW) complex that regulates anthocyanin accumulation. We determined that the hypomethylation of a CpG island in the CsAN1 promoter is associated with the purple phenotype. Furthermore, we demonstrated that low temperature and long illumination induced CsAN1 promoter demethylation, resulting in upregulated expression to promote anthocyanin accumulation in the foliage. The successful isolation of CsAN1 provides important information on the regulatory control of anthocyanin biosynthesis in C. sinensis and offers a genetic resource for the development of new varieties with enhanced anthocyanin content.

The effect of different degrees of drought stress on the aromatic constituents and their relative contents in fresh leaves of Lingtou Dancong tea plants was studied in this paper. The results showed that drought stress could evidently increase the kinds of aromatic components in the fresh leaves. The largest number of kinds (58) of the aromatic constituents was detected when soil relative water content was 53.90% among all the designed treatments, while the lowest number was found under a soil relative water content of 99.75%. The total amount of relative contents of 17 kinds of aromatic components such as linalool etc., increased with drought stress, whereas 12 kinds of aromatic components such as tetradecanoic acid etc., decreased with drought stress. Linalool, linalool oxide, tetradecane, 10-methylnonadecane, and dodecanal showed high contents under the soil relative water content of 53.90%; Cyclohexane, 1-hexadecene, and 1-tricosanolonly were induced in the soil relative water content of 53.90% and 29.25%; while drought stress could inhibit the synthesis of constituents of 7 kinds such as nonanedioic acid monomethyl ester, etc. Different degrees of drought stress could induce various kinds of aromatic constituents, and the number of aromatic constituents induced in fresh leaves increased with the strengthening of drought stress.

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The bottled milky tea in the market has been extracted and sterilized at high temperature resulting in poor aroma, favour deteriorated and other issues. In order to solve these problems, we added β-glucosidase(β-G) after high temperature sterilization and bottle-filling of milky tea beverage. It is proved to be scientific and efficiency of β-G in improving the aroma of milky tea beverage with black-tea, then the effect is the best when the concentration of β-G was 7.4 U/mL, and the amount of substrate was 100 mL, with 50 °C temperature and pH 5.6, as well as the reaction time is 2 hours. Sure, the innovation of this research is to obtain the effect principle and technology of enhancing aroma of milky tea through the treatment of exogenous β-G, which fundamentally solves the problem of serious loss of aroma air flow of milky tea, and ultimately realizes the maintenance of tea aroma after milky tea list and maximizes the maintenance of original tea flavour. Perhaps due to the improvement of milk tea quality, it can promote the sales of tea beverages.

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