The general aim of this study was to evaluate physicochemical properties, prebiotic activity and anticancer potential of jackfruit (Artocarpus heterophyllus) seed flour. The drying processes of jackfruit seeds were performed at 50, 60 and 70 °C in order to choose the optimal temperature for obtaining the flour based on drying time, polyphenol content and antioxidant capacity. The experimental values of the moisture ratio during jackfruit seed drying at different temperatures were obtained using Page’s equation to establish the drying time for the required moisture between 5 and 7% in the flour. The temperature of 60 °C was considered adequate for obtaining good flour and for performing its characterization. The chemical composition, total dietary fiber, functional properties and antioxidant capacity were then examined in the flour. The seed flour contains carbohydrates (73.87 g/100 g), dietary fiber (31 g/100 g), protein (14 g/100 g) and lipids (1 g/100 g). The lipid profile showed that the flour contained monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids. Sucrose, glucose, and fructose were found to be the predominant soluble sugars, and non-digestible oligosaccharides like 1-kestose were also found. The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively. The obtained flour exhibited good functional properties, such as water and oil absorption capacity, swelling power and emulsifier capacity. Additionally, this flour had a protective and preventive effect which is associated with the potential prebiotic activity in Lactobacillus casei and Bifidobacterium longum. These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.

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The aim of this study was to analyze the effect of diabetes mellitus (DM), acting through protein kinase C (PKC), on vascular tone associated to BKCa channel activity in rat mesenteric artery.MethodsDM was induced in male Wistar rats by intravenous injection of streptozotocin (STZ, 60mg/kg) and mesenteric artery rings were isolated for isometric force studies. The effects of PKC inhibitor Bisindolymaleimide I (Bis 1) and BKCa channel activator NS 1619 on phenylephrine induced contractions were evaluated and expressed as % force.ResultsNS1619 decreased maximal force in control but not in STZ rats (97.4 ± 16 % vehicle v.s. 81.6 ± 25% NS1619, p=0.011, n=7 for ctrl; 84 ± 13% v.s. 71.8 ± 36%, p=0.54, n = 6 for STZ). Bis1 decreased phenylephrine contraction in both control and STZ rats (61.8 ± 33%, p= 0.003, n =8 ctrl; 34 ± 12%, p=0.0001, n=6 for STZ); although inhibition was more prominent in STZ than in ctrl rats (p= 0.005). Relaxation due to nifedipine was higher for STZ (42±15% for ctrl v.s. 19.5 ±17 for STZ, p=0.031, n=6).ConclusionsDM leads to elevated PKC and VOCC activity, probably through BKCa channel inhibition which is not reversible by NS1619.Supported by: C12‐FAI‐03–60.60, UASLP