The West-African neighbouring Gambia and Saloum estuaries function in different ways, the first being normal (saltier waters in the lower river, salinity range 0-38) and the second being inverse (saltier waters in the upper river, salinity range 36-90). The common cichlid species Sarotherodon melanotheron was collected in both systems over 16 months (June 2001 to September 2002) at five locations. We quantified reproductive traits from macroscopic examination of the gonads, oocyte counts and measurements, and growth from interpretation and measurements of an otolith sub-sample. There was a clear seasonal cycle of reproduction in the estuaries with a peak at the beginning of the wet season (May-July). Size at maturation was smaller in the more saline environment, the Saloum, for both females (170 mm in Gambia and 131 mm in Saloum) and males (162 and 113 mm respectively). The relative fecundity was lower and the oocyte size was bigger in the freshwater location of the Gambia river. Growth, estimated by counting the annual opaque zones on whole otoliths, was reduced in the hypersaline environment of the Saloum (L ∞ = 198 mm, K = 0.26). Differences are less obvious between the growth in the Gambia (L ∞ = 238 mm, K = 0.21) and that in the Saloum with a salinity of less than 60 (L ∞ = 244 mm, K = 0.22). All these observations suggest that S. melanotheron is able to withstand saltier environments by limiting its growth, reducing the size-at-maturity, and changing its fecundity, but the most profound changes are only visible in hypersaline conditions.

International audienceThe influence of salinity on life-history traits was tested using two adjoining West African estuaries: the Gambia with a 'normal' salinity gradient (salinity always <40 and decreasing from the mouth upstream) and the Sine Saloum (Senegal) with an inverse gradient (from 35 at the estuary mouth up to >130 in the upper reaches). The breeding seasons and subsequent fork length (LF) at first maturity (LF50) were estimated for different fish species reproducing both in the Sine Saloum and in the Gambia River estuaries using a database built from experimental fish samplings between 1990 and 2003 with a purse seine (30 locations in the Sine Saloum and 44 in the Gambia). The database contained 30 553 individuals belonging to 60 different species among which only 20 species reproduced in both estuaries. The breeding seasons peaked just before, or at the beginning of the rainy season (June to July), and there were almost no sexually mature fishes at the beginning of the dry season (November to December). Patterns of differences between LF50 of the two estuaries did not follow a general trend (positive or negative), but varied in the same way for females and males of a given species. The LF50 was only systematically reduced with increasing salinity in species living in high-salinity waters (>70). For species living below 70, differences in sizes at first maturity between the two estuaries did not show any clear relationship with salinity. The smallest mature individual found in an environment was a good indicator of the size at first maturity reached in a particular ecosystem because the trend of the species differences between the two ecosystems generally followed that of the differences in size at maturity

The present paper describes the spermiogenesis and the ultrastructure of the spermatozoon of Fasciola gigantica, as revealed by transmission electron microscopy. Spermiogenesis in F. gigantica begins with the formation of a differentiation zone containing 2 centrioles with associated striated roots and an intercentriolar body between them. Each centriole develops a flagellum. Proximodistal fusion of these flagella with the median cytoplasmic extension gives rise to the spermatozoon. Spermiogenesis in F. gigantica is characterized by the formation of a dorsolateral cytoplasmic expansion, an external ornamentation of the cell membrane, and spinelike bodies. These 3 structures were also observed in the anterior part of the spermatozoon. Our study describes for the first time the simultaneous presence of dorsolateral cytoplasmic expansion, external ornamentation of the plasma membrane, and spinelike bodies in the spermatozoon of a trematode.

This work describes the first ultrastructural results on spermiogenesis and on the mature spermatozoon of Dicrocoelium hospes (Trematoda, Digenea) collected in Bos indicus from Senegal (Africa). Examination of this species was processed by TEM. Spermiogenesis follows the general pattern found in the digenean, but reveals a particularity consisting of the appearance of glycogen granules in the late spermatids within the testes. The mature spermatozoon possesses five distinct regions and presents all features found in Digenea gametes: two axonemes, mitochondria, nucleus and parallel cortical microtubules. However, several characters allow us to distinguish D. hospes from other digenetic trematodes within the Dicrocoeliidae family. In fact, we observed several structures that are absent in the other species of Dicrocoeliidae studied until now, such as: a cytoplasmic expansion, extramembranar ornamentation, spine-like bodies and two parallel mitochondria in the mature sperm. Moreover, additional particular characteristics were observed in this species in both extremities of the spermatozoon. This work produced new data on the ultrastructure of this trematode family which may be useful for phylogenetic purposes.

To our knowledge, this is the first ultrastructural study on spermiogenesis and the spermatozoon of a trematode belonging to the family Notocotylidae, Notocotylus neyrai. Spermiogenesis begins with the formation of the zone of differentiation which comprises striated rootlets associated with the two centrioles and an intercentriolar body in-between. It is characterised by an asynchronic flagellar rotation and subsequent proximodistal fusion with a median cytoplasmic process. The migration of the nucleus toward the median cytoplasmic process before its fusion with the free flagella is also described. The mature spermatozoon of N. neyrai is filiform and tapered at both ends and presents all the features found in the Digenea gamete: two axonemes, mitochondrion, nucleus and two bundles of parallel cortical microtubules. Nevertheless, several characters allow us to distinguish N. neyrai from other digenetic trematodes.