Significance and Impact of the Study: Due to the higher lethality effect of atmospheric cold plasma (ACP) on bacteria at the highest voltages (above 80 kV), only a few attempts have been made to investigate the ACP effect on injury induction in chlorine-adapted bacteria. Salmonella enterica is considered predominant in fresh-cut vegetable outbreaks. In the present study, results indicated that ACP application on chlorine-adapted S. enterica cells induced a higher percentage of injury than non-chlorine adapted at high voltages. Results suggested using lower voltages (6-8 kV) in the hurdle technology composed of chlorine disinfectants and ACP application.