Background and Aim: In this study FSH, LH, Testosterone, Estrogen, Progesterone serum levels in women affected by trigeminal neuralgia have been evaluated.Materials and Methods: This study is a cross sectional study during 2017-2018 in which FSH, LH, Testosterone, Estrogen, Progesterone serum levels in women affected by trigeminal neuralgia, who had referred to Emam Reza clinic and Oral and Maxillofacial Disease Department of Shiraz Dental Faculty, have been evaluated. Twenty-six women with trigeminal neuralgia were recruited in trigeminal neuralgia(TN) group and 26 healthy women whom their age were matched with TN group were enrolled in the healthy control group. Data was analyzed by SPSS version 18.Results: Sex hormone serum level was not significantly different between patients with TN and healthy control group (P value ≥0.05). In spite of this finding, the serum level of FSH in non-menopausal (P value=0.002) participants and progesterone in menopausal (P value=0.016) participants of TN and healthy control group, were significantly different. The serum level for both of these hormones were higher in patients with TN. In contrast to healthy control group, the sex hormone profiles of patients with TN, except LH did not follow the natural pattern changes based on menopausal status. Conclusion: In spite of no significant differences in sex hormonal profile of patients with TN and healthy controls, some hormonal disturbance in FSH and progesterone have been detected in TN patients in comparison between non-menopause and menopausal sex hormones profile.