Fabricating graphite electrode corrected with nanofiber by electrospinning as a considerable procedure for utilization in the fluid materials, milk, and syrup for detection of T2 mycotoxin is a significant technique. The modern biosensor was fabricated at normal degrees of room and utilized via buffer Britton–Robinson (B‐R) in pH = 5 to refine the chemico‐mechanical specifications. The electrochemical manner of the modified surface was surveyed using the scanning electron microscopy (SEM), cyclic voltammetry (CV), square wave voltammetry (SQWV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV). The corrected electrode displayed a linear reply to T2 toxin in two distinct concentration ranges of 30–100 nM with correlation coefficients of 0.99. The greatest signals in the square wave spectrums for the B‐R buffer created on the uttermost signals of the obtained streams were pH = 5 and 0.5 M of KNO3 for T2 toxin. The modified electrode has a big signal, broad dynamic concentration and high sensitivity and selectivity.