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It is more acceptable to believe that natural remedies are safer than synthetic subjects that with fewer side effects. The global market demand was increasing due to fusion of herbs. Standardization parameters and evaluation of herbal formulation is essential in order to assess the quality of drug of therapeutic value. This study was carried out in order to determine the bioactive compounds present in the marketed herbal formulation pykure capsule by using the GC–MS instrument. The extract was prepared by using 20 capsules by removing the capsule shell with ethanol solvent by simple maceration method. Capsule extract was analyzed by using Agilent 6890 GC with 59739N MSD and GC -MS equipped with Elite–I fused with silica capillary column (Cpsil 8cb:30mm×25mm×0.25mm). The result of GC-MS analysis confirmed the presence of 74 compounds. Keywords: Pykure capsule, Ethanolic extract, GC-MS analysis.

To evaluate the mosquito larvicidal activity on the ethanolic extract of Grewia flavescens juss (EEGF). Whole plant against Culex quinquefasciatus larvae. The larval mortality of 4th instar larvae of Culex quinquefasciatus after 24h and 48h of treatment which were tested separately in control and five test concentrations of 125 ppm, 250 ppm, 500 ppm,750 ppm, 1000 ppm concentration. The plant extract was screened to identify phytochemical bioactive compounds like alkaloids, flavonoids, saponins, tannins and glycosides. Different concentration of crude ethanolic extract of Grewia flavescensjuss. whole plant effectively showed larvicidal activity on Culex quinquefasciatus larvae. Based on the probit analysis values for 24h and 48h ethanolic extract of Grewia flavescens shows pronounced larvicidal activity. The LC50 and LC90 values of EEGF against mosquito 4th instar larvae of Culex quinquefasciatus was found 223.87 ppm and 724.43 ppm at 24hrs and for 48hrs the LC50 and LC90 values was found to be 181.97ppm and 374.76 ppm respectively. The preliminary phytochemical was performed in search of plants secondary metabolites which might be responsible for the larvicidal activity. The bioactive phytochemical classes which were identified and confirmed as alkaloids, flavonoids, saponins, tannins and glycosides. The ethanolic extract of this plant showed potent larvicidal activity and can be considered for further investigation. This experimental study was a pioneer attempt to establish Grewia flavescens juss. as a valuable resource of effective target specific natural mosquito larvicide. Keywords: Culex quinquefasciatus, larvicidal activity, ethanolic ext., phytochemical.

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