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Plant growth promoting rhizobacteria (PGPR) are a heterogeneous group of bacteria that can be found in the rhizosphere, which can improve the extent or quality of plant growth directly or indirectly. However, screening strategies for selecting the best rhizobacterial strain for rhizosphere competence with other microbial species in the plant rhizosphere will require more comprehensive knowledge. In present investigation nine different strains were tested for their PGPR properties by using RFLP analysis on 16S rRNA gene or amplified rDNA restriction analysis (ARDRA). 16 S rDNA amplification was done and restriction profiling was done using two endonuclease i.e. msp1 and taq1. Depending upon banding pattern of all the nine strains dendogram was created using NTsys software. A clear cut difference was seen in genetic diversity among the strains. Pseudomonas was found to be the most effective strain among all. Depending upon the outcome we can conclude that ARDRA can be effective tool for analyzing the genetic diversity among different bacteria and PGPR starin e.g. Pseudomonas, Bacillus can be used as a potent biofertilizer. Highlights •Nine Bacterial strains were obtained from Rhizospheric lab and were molecular characterized and sequenced.• ARDRA analysis of the diversity of strains revealed that strains are contained in two clusters,• Isolate P3 (Pseudomonas fluorescence) was found to be most efficient PGPR which solubilized insoluble phosphorus, produced IAA, siderophore, amylase, lipase and catalase.• A dendogram was constructed based on the restriction profile of nine strain restricted with Msp I and Taq I, revealed 14B, 8B, 9F and 13B had 100% similarity while strain 1B was placed distinctly in the tree (exhibiting less than 65% similarity).

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The electrochemical biosensor was designed for label-free detection of bovine serum albumin (BSA). In the developed electrochemical sensor gold coated polycarbonate membrane of different 30, 50 and 100 nm pore sizes. were used for detection. The gold coated polycarbonate membranes were thiolated by 16-Mercaptohexadecanoic acid (MHDA) and then activated by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) for the binding of the antibody (BSA). After activation of the membranes, the BSA antibody was immobilized and the membranes were subjected to the detection of BSA antigen with the help of a homemade electrochemical setup. The specifi city of the antibody was cross-checked with a non-corresponding Prostate Specifi c Antigen (PSA). The specifi city and sensitivity of the designed biosensor along with the signal amplifi cation due to binding of antigen-gold nanoparticle conjugate was also determined. The impedance corresponding to each step of membrane modifi cation was observed and it was found that there was a sharp increase in the measured impedance from modifi cation to detection. Also, there was multiple fold increase in signal due to tagging of GNP. The impedance corresponding to different pore sized membranes was used to fi nd a suitable pored membrane for the sensitive detection and it was observed that 30 nm pore size membrane showed comparatively better result than 50 and 100 nm pore size membrane.